O A Trentz1,2, D Arikketh3, V Sentilnathan3, S Hemmi4, A E Handschin4, B de Rosario3, P Mohandas3, P V A Mohandas3. 1. MIR (MIOT Institute of Research), MIOT Hospitals, Chennai, India. omanach@yahoo.com. 2. , Klusweg 18, 8032, Zurich, Switzerland. omanach@yahoo.com. 3. MIR (MIOT Institute of Research), MIOT Hospitals, Chennai, India. 4. Institute of Clinical Research, University Hospital Zurich, Zurich, Switzerland.
Abstract
INTRODUCTION: In adipose tissue-derived osteogenic cells (ADOC), osteoblast markers and surface proteins were determined and compared with osteoblasts harvested from cancellous bone (OB). METHOD: Osteocalcin (OC), core binding factor 1 (CBFA1), collagen type 1 (Coll1), alkaline phosphatase (ALP), nucleostemin (NS), and surface proteins CD 10, CD44, CD 59 and CD 105 were analyzed using RT-PCR, immunofluorescence and Western blot. RESULTS: Osteocalcin expression was more distinct in OB than in ADOC, but the other markers and surface proteins showed no differences. CONCLUSION: These data support the use of adipose tissue for future regenerative medicine; however, further studies are necessary to establish the role of long-term differentiation.
INTRODUCTION: In adipose tissue-derived osteogenic cells (ADOC), osteoblast markers and surface proteins were determined and compared with osteoblasts harvested from cancellous bone (OB). METHOD:Osteocalcin (OC), core binding factor 1 (CBFA1), collagen type 1 (Coll1), alkaline phosphatase (ALP), nucleostemin (NS), and surface proteins CD 10, CD44, CD 59 and CD 105 were analyzed using RT-PCR, immunofluorescence and Western blot. RESULTS:Osteocalcin expression was more distinct in OB than in ADOC, but the other markers and surface proteins showed no differences. CONCLUSION: These data support the use of adipose tissue for future regenerative medicine; however, further studies are necessary to establish the role of long-term differentiation.
Authors: Patricia A Zuk; Min Zhu; Peter Ashjian; Daniel A De Ugarte; Jerry I Huang; Hiroshi Mizuno; Zeni C Alfonso; John K Fraser; Prosper Benhaim; Marc H Hedrick Journal: Mol Biol Cell Date: 2002-12 Impact factor: 4.138
Authors: Jane B Lian; Amjad Javed; S Kaleem Zaidi; Christopher Lengner; Martin Montecino; Andre J van Wijnen; Janet L Stein; Gary S Stein Journal: Crit Rev Eukaryot Gene Expr Date: 2004 Impact factor: 1.807
Authors: Nadiya M Teplyuk; Mario Galindo; Viktor I Teplyuk; Jitesh Pratap; Daniel W Young; David Lapointe; Amjad Javed; Janet L Stein; Jane B Lian; Gary S Stein; Andre J van Wijnen Journal: J Biol Chem Date: 2008-07-14 Impact factor: 5.157
Authors: Omana A Trentz; Simon P Hoerstrup; Li K Sun; Lukas Bestmann; Andreas Platz; Otmar L Trentz Journal: Biomaterials Date: 2003-09 Impact factor: 12.479
Authors: C Chenu; S Colucci; M Grano; P Zigrino; R Barattolo; G Zambonin; N Baldini; P Vergnaud; P D Delmas; A Z Zallone Journal: J Cell Biol Date: 1994-11 Impact factor: 10.539
Authors: Paola Mina-Osorio; Beata Winnicka; Catherine O'Conor; Christina L Grant; Lotte K Vogel; Daniel Rodriguez-Pinto; Kathryn V Holmes; Enrique Ortega; Linda H Shapiro Journal: J Leukoc Biol Date: 2008-05-21 Impact factor: 4.962
Authors: Andy T Kwok; Joseph E Moore; Samuel Rosas; Bethany A Kerr; Rachel N Andrews; Callistus M Nguyen; Jingyun Lee; Cristina M Furdui; Boyce E Collins; Michael T Munley; Jeffrey S Willey Journal: Radiat Res Date: 2019-03-29 Impact factor: 2.841
Authors: Chirayu Patel; Lihong Shi; John F Whitesides; Brittni M Foster; Roberto J Fajardo; Ellen E Quillen; Bethany A Kerr Journal: Curr Protoc Date: 2022-03
Authors: Anna-Klara Amler; Patrick H Dinkelborg; Domenic Schlauch; Jacob Spinnen; Stefan Stich; Roland Lauster; Michael Sittinger; Susanne Nahles; Max Heiland; Lutz Kloke; Carsten Rendenbach; Benedicta Beck-Broichsitter; Tilo Dehne Journal: Int J Mol Sci Date: 2021-01-14 Impact factor: 5.923