Ivana Maldonado1, Alicia Arechavala2, Liliana Guelfand3, Silvia Relloso4, Claudia Garbasz5. 1. Laboratorio de Microbiología, Hospital Alemán, Buenos Aires, Argentina. 2. Unidad Micología, Hospital de Infecciosas F. J. Muñiz, Buenos Aires, Argentina. Electronic address: aliarecha@hotmail.com. 3. Sección Microbiología, Hospital General de Agudos J. A. Fernández, Buenos Aires, Argentina. 4. Laboratorio de Bacteriología, Micología y Parasitología, Centro de Educación Médica e Investigaciones Clínicas Norberto Quirno (CEMIC), Buenos Aires, Argentina. 5. Sección Microbiología, Hospital General de Agudos I. Pirovano, Buenos Aires, Argentina.
Abstract
BACKGROUND: Urinary tract infections are a frequent ailment in patients in intensive care units. Candida and other yeasts cause 5-12% of these infections. The value of the finding of any yeast is controversial, and there is no consensus about which parameters are adequate for differentiating urinary infections from colonization or contamination. AIMS: To analyse the epidemiological characteristics of patients with funguria, to determine potential cut-off points in cultures (to distinguish an infection from other conditions), to identify the prevalent yeast species, and to determine the value of a second urine sample. METHODS: A multicentre study was conducted in intensive care units of 14 hospitals in the Buenos Aires City Mycology Network. The first and second samples of urine from every patient were cultured. The presence of white cells and yeasts in direct examination, colony counts, and the identification of the isolated species, were evaluated. RESULTS: Yeasts grew in 12.2% of the samples. There was no statistical correlation between the number of white cells and the fungal colony-forming units. Eighty five percent of the patients had indwelling catheters. Funguria was not prevalent in women or in patients over the age of 65. Candida albicans, followed by Candida tropicalis, were the most frequently isolated yeasts. Candida parapsilosis and Candida glabrata appeared less frequently. The same species were isolated in 70% of second samples, and in 23% of the cases the second culture was negative. CONCLUSIONS: It was not possible to determine a useful cut-off point for colony counts to help in the diagnosis of urinary infections. As in other publications, C. albicans, followed by C. tropicalis, were the most prevalent species.
BACKGROUND: Urinary tract infections are a frequent ailment in patients in intensive care units. Candida and other yeasts cause 5-12% of these infections. The value of the finding of any yeast is controversial, and there is no consensus about which parameters are adequate for differentiating urinary infections from colonization or contamination. AIMS: To analyse the epidemiological characteristics of patients with funguria, to determine potential cut-off points in cultures (to distinguish an infection from other conditions), to identify the prevalent yeast species, and to determine the value of a second urine sample. METHODS: A multicentre study was conducted in intensive care units of 14 hospitals in the Buenos Aires City Mycology Network. The first and second samples of urine from every patient were cultured. The presence of white cells and yeasts in direct examination, colony counts, and the identification of the isolated species, were evaluated. RESULTS:Yeasts grew in 12.2% of the samples. There was no statistical correlation between the number of white cells and the fungal colony-forming units. Eighty five percent of the patients had indwelling catheters. Funguria was not prevalent in women or in patients over the age of 65. Candida albicans, followed by Candida tropicalis, were the most frequently isolated yeasts. Candida parapsilosis and Candida glabrata appeared less frequently. The same species were isolated in 70% of second samples, and in 23% of the cases the second culture was negative. CONCLUSIONS: It was not possible to determine a useful cut-off point for colony counts to help in the diagnosis of urinary infections. As in other publications, C. albicans, followed by C. tropicalis, were the most prevalent species.
Authors: G Jiménez-Guerra; I Casanovas Moreno-Torres; M Gutiérrez-Soto; F Vazquez-Alonso; A Sorlózano-Puerto; J M Navarro-Marí; J Gutiérrez-Fernández Journal: Rev Esp Quimioter Date: 2018-06-20 Impact factor: 1.553
Authors: Danny Ellen Meireles Leme; Allan Belarmino Rodrigues; Adriana Araújo de Almeida-Apolonio; Fabiana Gomes da Silva Dantas; Melyssa Fernanda Norman Negri; Terezinha Inez Estivalet Svidzinski; Jonas da Silva Mota; Claudia Andrea Lima Cardoso; Kelly Mari Pires de Oliveira Journal: Evid Based Complement Alternat Med Date: 2017-12-11 Impact factor: 2.629