Ruirui Dong1, Hui Liu2. 1. College of Medical Laboratory, Dalian Medical University, Dalian 11604, China. 2. College of Medical Laboratory, Dalian Medical University, Dalian 11604, China. Electronic address: immunology@dlmedu.edu.cn.
Abstract
OBJECTIVE: To establish a simple, stable method for measuring total complement activity in plasma. METHODS: Total complement activity (TCA) of plasma was measured using a classical method (CH50 method) and a self-hemolysis colorimetric method (new method). Human red blood cells (RBCs) were used as a hemolysis indicator system and rabbit-anti-human RBC antibody instead of the traditional hemolysin (rabbit-anti-sheep RBC antibody) in the new method. TCA in intensive care unit (ICU) patients and healthy individuals was measured using the new method. RESULTS: TCA using the new method and the CH50 method were significantly correlated. TCA in ICU patients was significantly lower than that in healthy individuals. CONCLUSION: The self-hemolysis colorimetric method is a simple and stable method, and has potential value in clinical applications.
OBJECTIVE: To establish a simple, stable method for measuring total complement activity in plasma. METHODS: Total complement activity (TCA) of plasma was measured using a classical method (CH50 method) and a self-hemolysis colorimetric method (new method). Human red blood cells (RBCs) were used as a hemolysis indicator system and rabbit-anti-human RBC antibody instead of the traditional hemolysin (rabbit-anti-sheep RBC antibody) in the new method. TCA in intensive care unit (ICU) patients and healthy individuals was measured using the new method. RESULTS:TCA using the new method and the CH50 method were significantly correlated. TCA in ICU patients was significantly lower than that in healthy individuals. CONCLUSION: The self-hemolysis colorimetric method is a simple and stable method, and has potential value in clinical applications.