AIMS: The study aimed to perform a systematic investigation of the effects of quercetin on biofilm formation and virulence factors in Pseudomonas aeruginosa. METHODS AND RESULTS: The Ps. aeruginosa strain PAO1 was selected as the test strain. The results indicated that quercetin did not impact the growth of PAO1 as determined by MIC and growth curve analysis. However, this compound significantly inhibited (P < 0·05) biofilm formation and production of virulence factors including pyocyanin, protease and elastase at a lower concentration than those for most previously reported plant extracts and substances. Considering the central role of quorum sensing (QS) in the regulation of biofilm and virulence factor, we further detected the transcriptional changes associated with QS and found that the expression levels of lasI, lasR, rhlI and rhlR were significantly reduced (P < 0·05) by 34, 68, 57 and 50%, respectively, in response to 16 μg ml(-1) quercetin. CONCLUSIONS: This study indicated that quercetin is an effective inhibitor of biofilm formation and virulence factors in Ps. aeruginosa. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study to demonstrate that quercetin is an effective inhibitor of QS, biofilm formation and virulence factors in Ps. aeruginosa. Furthermore, quercetin might have potential in fighting biofilm-related infections.
AIMS: The study aimed to perform a systematic investigation of the effects of quercetin on biofilm formation and virulence factors in Pseudomonas aeruginosa. METHODS AND RESULTS: The Ps. aeruginosa strain PAO1 was selected as the test strain. The results indicated that quercetin did not impact the growth of PAO1 as determined by MIC and growth curve analysis. However, this compound significantly inhibited (P < 0·05) biofilm formation and production of virulence factors including pyocyanin, protease and elastase at a lower concentration than those for most previously reported plant extracts and substances. Considering the central role of quorum sensing (QS) in the regulation of biofilm and virulence factor, we further detected the transcriptional changes associated with QS and found that the expression levels of lasI, lasR, rhlI and rhlR were significantly reduced (P < 0·05) by 34, 68, 57 and 50%, respectively, in response to 16 μg ml(-1) quercetin. CONCLUSIONS: This study indicated that quercetin is an effective inhibitor of biofilm formation and virulence factors in Ps. aeruginosa. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study to demonstrate that quercetin is an effective inhibitor of QS, biofilm formation and virulence factors in Ps. aeruginosa. Furthermore, quercetin might have potential in fighting biofilm-related infections.
Authors: Jon E Paczkowski; Sampriti Mukherjee; Amelia R McCready; Jian-Ping Cong; Christopher J Aquino; Hahn Kim; Brad R Henke; Chari D Smith; Bonnie L Bassler Journal: J Biol Chem Date: 2017-01-24 Impact factor: 5.157
Authors: Wen Yin; Siyang Xu; Yiting Wang; Yuling Zhang; Shan-Ho Chou; Michael Y Galperin; Jin He Journal: Crit Rev Microbiol Date: 2020-12-28 Impact factor: 7.624
Authors: Hanan M Al-Yousef; Atallah F Ahmed; Nasser A Al-Shabib; Sameen Laeeq; Rais A Khan; Md T Rehman; Ali Alsalme; Mohamed F Al-Ajmi; Mohammad S Khan; Fohad M Husain Journal: Front Microbiol Date: 2017-09-05 Impact factor: 5.640