| Literature DB >> 26808153 |
Abstract
[This corrects the article DOI: 10.1371/journal.pgen.1005745.].Entities:
Year: 2016 PMID: 26808153 PMCID: PMC4726583 DOI: 10.1371/journal.pgen.1005832
Source DB: PubMed Journal: PLoS Genet ISSN: 1553-7390 Impact factor: 5.917
Fig 2Characterization of CaMKII proteins isolated under no-stress and Ca2+-stress conditions.
(A) C-terminal Flag-tagged proteins were purified from HEK293T cells and its purity examined by SDS-PAGE. Each sample is loaded in different amounts in two lanes. (B) Kinase activity assay of the proteins isolated from both conditions. Using the same amount of protein, CaMKII isolated under Ca2+ stress shows consistently higher activity than the protein isolated from no-stress condition. (C) Kinase activities of stressed and non-stressed samples at different substrate concentrations upon treatment with H2O2, or ATP (D).