Efser Oztas1, Sibel Ozler2, Aytekin Tokmak2, Nafiye Yilmaz2, Huseyin Tugrul Celik3, Fatmanur Hacıevliyagil Kazancı3, Nuri Danisman2, Merve Ergin4, Halil Ibrahim Yakut5. 1. Zekai Tahir Burak Women's Health Education and Research Hospital, Department of Obstetrics and Gynecology, Ankara, Turkey. Electronic address: efseroztas@gmail.com. 2. Zekai Tahir Burak Women's Health Education and Research Hospital, Department of Obstetrics and Gynecology, Ankara, Turkey. 3. Turgut Ozal University Faculty of Medicine, Department of Clinical Biochemistry, Ankara, Turkey. 4. Yildirim Beyazit University Faculty of Medicine, Department of Clinical Biochemistry, Ankara, Turkey. 5. Zekai Tahir Burak Women's Health Education and Research Hospital, Department of Pediatrics, Ankara, Turkey.
Abstract
OBJECTIVES: Our aim was to determine serum perforin and granzyme-B levels in adolescent PCOS patients, and to investigate whether they are associated with some of the insulin sensitivity, obesity and cardiovascular (CV) risk markers and metabolic syndrome. STUDY DESIGN: A case-control study was carried out including a total of 172 adolescents (83 PCOS patients and 89 age-matched healthy controls). Participants were recruited consecutively. Homeostasis model assessment (HOMA-IR), lipid parameters, and anthropometric measurements were determined. Serum perforin and granzyme B levels were measured by commercially available ELISA kits. HOMA-IR>3.16 was considered to indicate the presence of insulin resistance. Logistic regression analysis was applied for the predictive value of granzyme-B for increased CV risk in PCOS patients. RESULTS: As body mass index (BMI) of the PCOS patients was significantly higher than the controls (median 24.6kg/m(2) and 21.4kg/m(2), respectively, p<0.001) all parameters were evaluated after adjustment for BMI. Adolescents with PCOS had significantly higher levels of fasting glucose, insulin, HOMA-IR and granzyme-B when compared with controls. According to the results of logistic regression analysis, granzyme-B levels were found to be significantly associated with increased HOMA-IR (OR=6.120, 95% CI: 2.352-15.926, p<0.001) in adolescent PCOS patients. Additionally, elevated levels of serum granzyme-B were predictive for increased CV risk in PCOS patients (OR=0.237, 95% CI: 0.091-0.616, p=0.003). CONCLUSIONS: Increased levels of serum granzyme-B are independently associated with insulin resistance and also with increased CV risk in adolescent polycystic ovary syndrome patients.
OBJECTIVES: Our aim was to determine serum perforin and granzyme-B levels in adolescent PCOSpatients, and to investigate whether they are associated with some of the insulin sensitivity, obesity and cardiovascular (CV) risk markers and metabolic syndrome. STUDY DESIGN: A case-control study was carried out including a total of 172 adolescents (83 PCOSpatients and 89 age-matched healthy controls). Participants were recruited consecutively. Homeostasis model assessment (HOMA-IR), lipid parameters, and anthropometric measurements were determined. Serum perforin and granzyme B levels were measured by commercially available ELISA kits. HOMA-IR>3.16 was considered to indicate the presence of insulin resistance. Logistic regression analysis was applied for the predictive value of granzyme-B for increased CV risk in PCOSpatients. RESULTS: As body mass index (BMI) of the PCOSpatients was significantly higher than the controls (median 24.6kg/m(2) and 21.4kg/m(2), respectively, p<0.001) all parameters were evaluated after adjustment for BMI. Adolescents with PCOS had significantly higher levels of fasting glucose, insulin, HOMA-IR and granzyme-B when compared with controls. According to the results of logistic regression analysis, granzyme-B levels were found to be significantly associated with increased HOMA-IR (OR=6.120, 95% CI: 2.352-15.926, p<0.001) in adolescent PCOSpatients. Additionally, elevated levels of serum granzyme-B were predictive for increased CV risk in PCOSpatients (OR=0.237, 95% CI: 0.091-0.616, p=0.003). CONCLUSIONS: Increased levels of serum granzyme-B are independently associated with insulin resistance and also with increased CV risk in adolescent polycystic ovary syndromepatients.
Authors: Flavia Agata Cimini; Ilaria Barchetta; Valentina Ceccarelli; Caterina Chiappetta; Alberto Di Biasio; Laura Bertoccini; Federica Sentinelli; Frida Leonetti; Gianfranco Silecchia; Claudio Di Cristofano; Marco Giorgio Baroni; Francesca Velotti; Maria Gisella Cavallo Journal: Front Immunol Date: 2020-11-18 Impact factor: 7.561