Harun Egemen Tolunay1, Yavuz Emre Şükür1, Sinan Ozkavukcu1, Mehmet Murat Seval1, Can Ateş2, Vugar Ali Türksoy3, Tolga Ecemiş4, Cem Somer Atabekoğlu1, Batuhan Özmen1, Bülent Berker1, Murat Sönmezer5. 1. Ankara University School of Medicine, Department of Obstetrics and Gynecology, IVF Unit, Turkey. 2. Ankara University School of Medicine, Department of Biostatistics, Turkey. 3. Ankara University School of Medicine, Department of Forensic Sciences, Turkey. 4. Ankara Liv Hospital, Department of Obstetrics and Gynecology, IVF Unit, Turkey. 5. Ankara University School of Medicine, Department of Obstetrics and Gynecology, IVF Unit, Turkey. Electronic address: msonmezer@gmail.com.
Abstract
OBJECTIVES: To assess the effects of heavy metal and trace element concentrations in blood and follicular fluid on assisted reproductive technology cycle outcome. STUDY DESIGN: A prospective study was conducted between January 2012 and July 2012 in a university hospital infertility clinic. One hundred and one patients with unexplained infertility who underwent intracytoplasmic sperm injection using GnRH-antagonist protocol were recruited. Concentrations of four toxic metals (Cd, Pb, Hg, As) and three trace elements (Cu, Zn, Fe) were measured both in blood and follicular fluid specimens. Patients were evaluated in two groups; the study group consisted of patients with ongoing pregnancy (n=20) and the reference group consisted of patients experienced assisted reproductive technology failure, miscarriage or biochemical pregnancy (n=81). RESULTS: Demographics and cycle parameters were comparable between the groups except for median number of day 3 Grade A embryos. Statistically significant negative correlations were found between blood Pb levels and number of MII oocytes, implantation, clinical pregnancy and ongoing pregnancy rates. Results of the log binomial regression revealed 2.2% lower risk for ongoing pregnancy for each 1μg/dL higher blood Pb concentration while holding the other variables in the model constant (RR 0.978; 95% CI 0.956-0.998; P=.041). Also, the results revealed 71.9% lower risk for ongoing pregnancy for each 1μg/dL higher follicular fluid Cu concentration while holding the other variables in the model constant (RR 0.288; 95% CI 0.085-0.92; P=.039). CONCLUSION: Blood concentrations of Pb and follicular fluid concentrations of Cu seem to have significant impacts on assisted reproductive technology cycle outcome.
OBJECTIVES: To assess the effects of heavy metal and trace element concentrations in blood and follicular fluid on assisted reproductive technology cycle outcome. STUDY DESIGN: A prospective study was conducted between January 2012 and July 2012 in a university hospital infertility clinic. One hundred and one patients with unexplained infertility who underwent intracytoplasmic sperm injection using GnRH-antagonist protocol were recruited. Concentrations of four toxic metals (Cd, Pb, Hg, As) and three trace elements (Cu, Zn, Fe) were measured both in blood and follicular fluid specimens. Patients were evaluated in two groups; the study group consisted of patients with ongoing pregnancy (n=20) and the reference group consisted of patients experienced assisted reproductive technology failure, miscarriage or biochemical pregnancy (n=81). RESULTS: Demographics and cycle parameters were comparable between the groups except for median number of day 3 Grade A embryos. Statistically significant negative correlations were found between blood Pb levels and number of MII oocytes, implantation, clinical pregnancy and ongoing pregnancy rates. Results of the log binomial regression revealed 2.2% lower risk for ongoing pregnancy for each 1μg/dL higher blood Pb concentration while holding the other variables in the model constant (RR 0.978; 95% CI 0.956-0.998; P=.041). Also, the results revealed 71.9% lower risk for ongoing pregnancy for each 1μg/dL higher follicular fluid Cu concentration while holding the other variables in the model constant (RR 0.288; 95% CI 0.085-0.92; P=.039). CONCLUSION: Blood concentrations of Pb and follicular fluid concentrations of Cu seem to have significant impacts on assisted reproductive technology cycle outcome.
Authors: Juan Mateo Anchordoquy; Juan Patricio Anchordoquy; Noelia Nikoloff; Ana M Pascua; Cecilia C Furnus Journal: Environ Sci Pollut Res Int Date: 2017-07-11 Impact factor: 4.223
Authors: Aubrey L Galusha; Aubreian C Haig; Michael S Bloom; Pamela C Kruger; Alexandra McGough; Nikolaus Lenhart; Rebecca Wong; Victor Y Fujimoto; Evelyn Mok-Lin; Patrick J Parsons Journal: J Anal At Spectrom Date: 2019-02-25 Impact factor: 4.023
Authors: Celeste D Butts; Michael S Bloom; Alexandra McGough; Nikolaus Lenhart; Rebecca Wong; Evelyn Mok-Lin; Patrick J Parsons; Aubrey L Galusha; Richard W Browne; Recai M Yucel; Beth J Feingold; Victor Y Fujimoto Journal: Hum Reprod Open Date: 2021-07-29