| Literature DB >> 2680139 |
T H Flewett, C F Arias, L F Avendano, A Ghafoor, M M Mathan, L Mendis, K Moe, R F Bishop.
Abstract
Faeces obtained from 1,163 children (including 66 newborn babies) were analysed in parallel for the presence of rotavirus particles using two enzyme-linked immunosorbent assay kits. The kits had been formulated by the WHO Collaborating Centre for Reference and Research on Rotavirus (WHO-ELISA kit) and by DAKOPATTS (DAKO-ELISA kit) to be suitable for use in laboratories in developing countries. The kits were evaluated in laboratories in Burma, Chile, India, Mexico, Pakistan, Sri Lanka and the United Kingdom. Comparison of the results obtained with the two kits indicated that the DAKO-ELISA had an overall sensitivity of 97% and a specificity of 97% relative to the WHO-ELISA. In individual laboratories the DAKO-ELISA (K349) kit had a sensitivity in the range 90-100%, and a specificity of 85-100%. The kit showed a sensitivity of 100% and a specificity of 98% in assays on faeces obtained from newborn babies. We conclude that the DAKO-ELISA is as sensitive and specific as the WHO-ELISA for the detection of rotavirus antigen in faeces.Entities:
Keywords: Diarrhea; Diarrhea, Infantile; Diseases; Evaluation; Examinations And Diagnoses; International Agencies; Laboratory Examinations And Diagnoses; Laboratory Procedures; Organizations; Un; Viral Diseases; Who
Mesh：
Year: 1989 PMID： 2680139 PMCID： PMC2491265
Source DB: PubMed Journal: Bull World Health Organ ISSN： 0042-9686 Impact factor: 9.408