| Literature DB >> 26799742 |
Kendal Prill, Pamela Windsor Reid, Serene L Wohlgemuth, David B Pilgrim.
Abstract
Entities:
Year: 2016 PMID: 26799742 PMCID: PMC4723138 DOI: 10.1371/journal.pone.0148027
Source DB: PubMed Journal: PLoS One ISSN: 1932-6203 Impact factor: 3.240
Fig 3SMYD1b is required for fast myosin incorporation during sarcomere assembly.
At 48hpf, fast myosin (F310—green) and actin (phalloidin—red) staining is visible in the premyofibrils in wt zebrafish tails (A&B), while absent from the premyofibrils in sth fast muscle tissue (C&D). Slow muscle (F59) develops normally in both wild type and still heart zebrafish at 48hpf (E&F, G&H). At 19hpf, in wild type embryos, fast myosin (F310) is beginning to be incorporated into the maturing myofibril (I) and overlaps (white arrowheads) with the developing actin (phalloidin) (J) fibers in trunk muscle (K, merge, K’ inset, white arrowhead). Fast myosin is not incorporated into the maturing premyofibril (L), although actin fibers are still present (M&N, N’ inset).