| Literature DB >> 26797124 |
Qiang Li1, Rachel K Lex1, HaeWon Chung1, Simone M Giovanetti1, Zhicheng Ji2, Hongkai Ji2, Maria D Person3, Jonghwan Kim1, Steven A Vokes4.
Abstract
The Hedgehog (HH) signaling pathway is essential for the maintenance and response of several types of stem cells. To study the transcriptional response of stem cells to HH signaling, we searched for proteins binding to GLI proteins, the transcriptional effectors of the HH pathway in mouse embryonic stem (ES) cells. We found that both GLI3 and GLI1 bind to the pluripotency factor NANOG. The ectopic expression of NANOG inhibits GLI1-mediated transcriptional responses in a dose-dependent fashion. In differentiating ES cells, the presence of NANOG reduces the transcriptional response of cells to HH. Finally, we found thatGli1andNanogare co-expressed in ES cells at high levels. We propose that NANOG acts as a negative feedback component that provides stem cell-specific regulation of the HH pathway.Entities:
Keywords: GLI proteins; Hedgehog signaling pathway; Nanog; differentiation; embryonic stem cell; repressor; stem cells; transcription repressor
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Year: 2016 PMID： 26797124 PMCID： PMC4807297 DOI： 10.1074/jbc.M116.714857
Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157