Yuhua Shan1, Nan Shen2, Longzhi Han3, Qimin Chen4, Jianjun Zhang5, Xidai Long6, Qiang Xia7. 1. Department of Liver Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, China. Electronic address: 1474229057@qq.com. 2. Joint Molecular Rheumatology Laboratory of the Institute of Health Sciences and Shanghai Renji Hospital, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, and Shanghai Jiaotong University School of Medicine, Shanghai, China. 3. Department of Liver Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, China. Electronic address: longerhans@163.com. 4. Department of Surgery, Shanghai Children's Medical Centre, School of Medicine, Shanghai Jiao Tong University, China. Electronic address: chenqiminok@gmail.com. 5. Department of Liver Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, China. Electronic address: zhangjianjun@medmail.com.cn. 6. Department of Liver Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, China. Electronic address: longxidai@163.com. 7. Department of Liver Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, China. Electronic address: xiaqiang@shsmu.edu.cn.
Abstract
BACKGROUND: Single nucleotide polymorphisms within microRNAs are known to affect the risk in development and prognosis of many diseases. This study was designed to investigate whether polymorphism of microRNA-499 (miR-499, rs3746444 A>G) is associated with risk to biliary atresia (BA). METHODS: A hospital-based cases-control study was performed on a total of 507 Han Chinese (207 BA cases and 300 ethnically-matched healthy controls without any evidence of liver diseases) so as to analyze the association between miR-499 rs3746444 polymorphism and BA risk as well as liver function remission (LFR) after liver transplantation. RESULTS: A significant higher frequency of the rs3746444 G alleles was found in the BA cases than the control group (odd ratio, 1.55, 95% confidence intervals [CIs], 1.15-2.10). This polymorphism was also observed to correlate with some clinic-pathological features of BA cases such as liver inflammatory. Further research found both higher levels of IL-6 (P<0.05) and TNF-α (P<0.05) in removed liver as well as in serum. What is more, the miR-499 rs3746444 polymorphism significantly affected the status of LFR (hazard ratio, 1.37; 95% CI, 1.08-1.83). CONCLUSIONS: MiR-499 (rs3746444) gene polymorphisms may be genetic determinants for increased risk of BA and prolonged recovery of BA patients after liver transplantation in Han Chinese.
BACKGROUND: Single nucleotide polymorphisms within microRNAs are known to affect the risk in development and prognosis of many diseases. This study was designed to investigate whether polymorphism of microRNA-499 (miR-499, rs3746444 A>G) is associated with risk to biliary atresia (BA). METHODS: A hospital-based cases-control study was performed on a total of 507 Han Chinese (207 BA cases and 300 ethnically-matched healthy controls without any evidence of liver diseases) so as to analyze the association between miR-499rs3746444 polymorphism and BA risk as well as liver function remission (LFR) after liver transplantation. RESULTS: A significant higher frequency of the rs3746444 G alleles was found in the BA cases than the control group (odd ratio, 1.55, 95% confidence intervals [CIs], 1.15-2.10). This polymorphism was also observed to correlate with some clinic-pathological features of BA cases such as liver inflammatory. Further research found both higher levels of IL-6 (P<0.05) and TNF-α (P<0.05) in removed liver as well as in serum. What is more, the miR-499rs3746444 polymorphism significantly affected the status of LFR (hazard ratio, 1.37; 95% CI, 1.08-1.83). CONCLUSIONS:MiR-499 (rs3746444) gene polymorphisms may be genetic determinants for increased risk of BA and prolonged recovery of BA patients after liver transplantation in Han Chinese.
Authors: Lin He; Patrick Ho Yu Chung; Vincent Chi Hang Lui; Clara Sze Man Tang; Paul Kwong Hang Tam Journal: Int J Mol Sci Date: 2022-04-27 Impact factor: 6.208