Ming Chu1, Yao Gao1, Yanjuan Zhang1, Bin Zhou1, Bingruo Wu1, Jing Yao1, Di Xu1. 1. 1 Department of Geriatrics, 2 Department of Cardiology, First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, China ; 3 Departments of Genetics, Pediatrics and Medicine (Cardiology), Albert Einstein College of Medicine of Yeshiva University, New York, NY, USA.
Abstract
BACKGROUND: Sepsis-induced myocardial dysfunction is a common and severe complication of septic shock. Conventional echocardiography often fails to reveal myocardial depression in severe sepsis due to hemodynamic changes; in contrast, decline of strain measurements by speckle tracking echocardiography (STE) may indicate impaired cardiac function. This study investigates the role of STE in detecting lipopolysaccharide (LPS)-induced cardiac dysfunction with mouse models. METHODS: We evaluated cardiac function in 20 mice at baseline, 6 h (n=10) and 20 h (n=10) after LPS injection to monitor the development of heart failure induced by severe sepsis using 2-D and M-mode echocardiography. Ejection fraction (EF) and fractional shortening (FS) were measured with standard M-mode tracings, whereas circumferential and radial strain was derived from STE. Serum biochemical and cardiac histopathological examinations were performed to determine sepsis-induced myocardial injury. RESULTS: Left ventricular (LV) myocardial function was significantly reduced at 6 h after LPS treatment assessed by circumferential strain (-14.65%±3.00% to -8.48%±1.72%, P=0.006), whereas there were no significant differences between 6 and 20 h group. Conversely, EF and FS were significantly increased at 20 h when comparing to 6 h (P<0.05) accompanied with marked decreases in EF and FS 6 h following LPS administration. Consistent with strain echocardiographic results, we showed that LPS injection leaded to elevated serum level of cardiac Troponin-T (cTnT), CK-MB and rising leucocytes infiltration into myocardium within 20 h. CONCLUSIONS: Altogether, these results demonstrate that, circumferential strain by STE is a specific and reliable value for evaluating LPS-induced cardiac dysfunction in mice.
BACKGROUND:Sepsis-induced myocardial dysfunction is a common and severe complication of septic shock. Conventional echocardiography often fails to reveal myocardial depression in severe sepsis due to hemodynamic changes; in contrast, decline of strain measurements by speckle tracking echocardiography (STE) may indicate impaired cardiac function. This study investigates the role of STE in detecting lipopolysaccharide (LPS)-induced cardiac dysfunction with mouse models. METHODS: We evaluated cardiac function in 20 mice at baseline, 6 h (n=10) and 20 h (n=10) after LPS injection to monitor the development of heart failure induced by severe sepsis using 2-D and M-mode echocardiography. Ejection fraction (EF) and fractional shortening (FS) were measured with standard M-mode tracings, whereas circumferential and radial strain was derived from STE. Serum biochemical and cardiac histopathological examinations were performed to determine sepsis-induced myocardial injury. RESULTS: Left ventricular (LV) myocardial function was significantly reduced at 6 h after LPS treatment assessed by circumferential strain (-14.65%±3.00% to -8.48%±1.72%, P=0.006), whereas there were no significant differences between 6 and 20 h group. Conversely, EF and FS were significantly increased at 20 h when comparing to 6 h (P<0.05) accompanied with marked decreases in EF and FS 6 h following LPS administration. Consistent with strain echocardiographic results, we showed that LPS injection leaded to elevated serum level of cardiac Troponin-T (cTnT), CK-MB and rising leucocytes infiltration into myocardium within 20 h. CONCLUSIONS: Altogether, these results demonstrate that, circumferential strain by STE is a specific and reliable value for evaluating LPS-induced cardiac dysfunction in mice.
Authors: Sonali Basu; Lowell H Frank; Kimberly E Fenton; Craig A Sable; Richard J Levy; John T Berger Journal: Pediatr Crit Care Med Date: 2012-05 Impact factor: 3.624
Authors: Sergio L Zanotti Cavazzoni; Massimiliano Guglielmi; Joseph E Parrillo; Tracy Walker; R Phillip Dellinger; Steven M Hollenberg Journal: Chest Date: 2010-07-22 Impact factor: 9.410
Authors: Helge Røsjø; Marjut Varpula; Tor-Arne Hagve; Sari Karlsson; Esko Ruokonen; Ville Pettilä; Torbjørn Omland Journal: Intensive Care Med Date: 2010-10-12 Impact factor: 17.440
Authors: Yu Inata; Giovanna Piraino; Paul W Hake; Michael O'Connor; Patrick Lahni; Vivian Wolfe; Christine Schulte; Victoria Moore; Jeanne M James; Basilia Zingarelli Journal: Am J Physiol Heart Circ Physiol Date: 2018-07-06 Impact factor: 4.733
Authors: Deanna K Sosnowski; K Lockhart Jamieson; Artiom Gruzdev; Yingxi Li; Robert Valencia; Ala Yousef; Zamaneh Kassiri; Darryl C Zeldin; John M Seubert Journal: Am J Physiol Heart Circ Physiol Date: 2022-08-19 Impact factor: 5.125