| Literature DB >> 26787461 |
Xue Fu1,2,3,4, Xingjie Gao1,2,3,4, Lin Ge1,2,3,4, Xiaoteng Cui1,2,3,4, Chao Su1,2,3,4, Wendong Yang1,2,3,4, Xiaoming Sun1,2,3,4, Wei Zhang1,2,3,4, Zhi Yao1,2,3,4, Xi Yang5, Jie Yang1,2,3,4,5.
Abstract
Malonate, a classic inhibitor of respiratory electron transport chain, induces mitochondrial stress. Stress granules (SGs) are a kind of dynamic foci structure during stress. The study on the connection of mitochondrial stress and SG assembly is still limited. Here, we demonstrated that malonate treatment leads to SG formation and translation inhibition, apart from mitochondrial stress, including enhanced ROS formation, reduced mitochondrial Δψm and ATP level. The phosphorylation levels of eIF2α and 4EBP1 protein were affected upon mitochondrial dysfunction. However, knockdown of 4EBP1 affected SG formation, rather than eIF2α. In addition, an increase of ATP level under mitochondrial stress enhanced malonate-induced SG aggregation. Overall, malonate stimulation triggers mitochondrial stress and induces the assembly of non-canonical cellular SGs via 4EBP1 pathway.Entities:
Keywords: 4E binding protein 1; adenosine triphosphate; eukaryotic initiation factor 2 alpha; malonate; mitochondria; phosphorylation; stress granules
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Year: 2016 PMID： 26787461 DOI： 10.1002/1873-3468.12049
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124