Abuchahama Saifodine1, Janet Fyfe2, Aina Sievers3, Elizabeth Coelho4, Khalide Azam5, James Black6. 1. Faculdade de Medicina, Universidade Eduardo Mondlane, Mozambique. Electronic address: asaifodine@gmail.com. 2. Victorian Infectious Diseases Reference Laboratory, Melbourne, Australia. Electronic address: janet.Fyfe@mh.org.au. 3. Victorian Infectious Diseases Reference Laboratory, Melbourne, Australia. Electronic address: aina.sievers@mh.org.au. 4. Tuberculosis National Reference Laboratory, Ministry of Health, Mozambique. Electronic address: bethhamene@gmail.com. 5. Tuberculosis National Reference Laboratory, Ministry of Health, Mozambique. Electronic address: kie.azam@live.com. 6. Nossal Institute for Global Health, Melbourne School of Population and Global Health, The University of Melbourne, Australia. Electronic address: jim.black@unimelb.edu.au.
Abstract
BACKGROUND: Tuberculosis (TB) represents a serious public health problem in Mozambique, with an estimated incidence rate of 548 cases per 100,000 population in 2011. Information on the molecular epidemiology of Mycobacterium tuberculosis (MTB) strains circulating in Mozambique is limited. This study provides the first description of the genetic diversity of MTB strains circulating in Beira city, the second largest town in Mozambique. METHODS: A total of 67 MTB isolates were tested to determine genetic lineages and diversity. The genetic lineages were determined using real-time PCR while genetic diversity was assessed by obtaining Mycobacterial Interspersed Repetitive Unit-Variable Numbers of Tandem Repeat profiles. RESULTS: Only three of the six major lineages were represented, with 41 (61%) strains belonging to lineage 1, 25 (37%) belonging to lineage 4 and the remaining isolate belonging to lineage 3. No lineage 2 strains (containing the Beijing family) were identified. A high degree of diversity amongst the strains from both lineages 1 and 4 were observed. Comparison of the profiles of representative strains with those of reference strains in the MIRU-VNTRplus database revealed that all lineage 1 isolates clustered with the Eastern African Indian (EAI) 5 sub-family. The lineage 4 strains clustered with a variety of different sub-family strains, including the Latin-American-Mediterranean (LAM) 1 sub-family, the Haarlem, Uganda 1 and Cameroon sub-families and the T2-S sub-family. CONCLUSIONS: The TB epidemic in Beira city is caused by a diverse group of MTB strains predominantly belonging to lineages 1 and 4.
BACKGROUND:Tuberculosis (TB) represents a serious public health problem in Mozambique, with an estimated incidence rate of 548 cases per 100,000 population in 2011. Information on the molecular epidemiology of Mycobacterium tuberculosis (MTB) strains circulating in Mozambique is limited. This study provides the first description of the genetic diversity of MTB strains circulating in Beira city, the second largest town in Mozambique. METHODS: A total of 67 MTB isolates were tested to determine genetic lineages and diversity. The genetic lineages were determined using real-time PCR while genetic diversity was assessed by obtaining Mycobacterial Interspersed Repetitive Unit-Variable Numbers of Tandem Repeat profiles. RESULTS: Only three of the six major lineages were represented, with 41 (61%) strains belonging to lineage 1, 25 (37%) belonging to lineage 4 and the remaining isolate belonging to lineage 3. No lineage 2 strains (containing the Beijing family) were identified. A high degree of diversity amongst the strains from both lineages 1 and 4 were observed. Comparison of the profiles of representative strains with those of reference strains in the MIRU-VNTRplus database revealed that all lineage 1 isolates clustered with the Eastern African Indian (EAI) 5 sub-family. The lineage 4 strains clustered with a variety of different sub-family strains, including the Latin-American-Mediterranean (LAM) 1 sub-family, the Haarlem, Uganda 1 and Cameroon sub-families and the T2-S sub-family. CONCLUSIONS: The TB epidemic in Beira city is caused by a diverse group of MTB strains predominantly belonging to lineages 1 and 4.
Authors: Bourahima Kone; Anou M Somboro; Jane L Holl; Bocar Baya; Antieme Acg Togo; Yeya Dit Sadio Sarro; Bassirou Diarra; Ousmane Kodio; Robert L Murphy; William Bishai; Mamoudou Maiga; Seydou Doumbia Journal: Int J Mol Epidemiol Genet Date: 2020-06-15
Authors: Evangelina Inacio Namburete; Anzaan Dippenaar; Emilyn Costa Conceição; Cinara Feliciano; Margarida Maria Passeri do Nascimento; Kamila Chagas Peronni; Wilson Araújo Silva; Josefo João Ferro; Lee H Harrison; Robin Mark Warren; Valdes Roberto Bollela Journal: Tuberculosis (Edinb) Date: 2020-01-29 Impact factor: 2.973
Authors: João Perdigão; Carla Silva; Jaciara Diniz; Catarina Pereira; Diana Machado; Jorge Ramos; Hugo Silva; Fernanda Abilleira; Clarice Brum; Ana J Reis; Maíra Macedo; João L Scaini; Ana B Silva; Leonardo Esteves; Rita Macedo; Fernando Maltez; Sofia Clemente; Elizabeth Coelho; Sofia Viegas; Paulo Rabna; Amabélia Rodrigues; Nuno Taveira; Luísa Jordao; Afrânio Kritski; José R Lapa E Silva; Igor Mokrousov; David Couvin; Nalin Rastogi; Isabel Couto; Arnab Pain; Ruth McNerney; Taane G Clark; Andrea von Groll; Elis R Dalla-Costa; Maria Lúcia Rossetti; Pedro E A Silva; Miguel Viveiros; Isabel Portugal Journal: Infect Genet Evol Date: 2018-03-17 Impact factor: 3.342