Jacqueline A Tickle1, Stuart I Jenkins1, Boris Polyak2, Mark R Pickard1, Divya M Chari1. 1. Institute for Science & Technology in Medicine, School of Medicine, David Weatherall Building, Keele University, Staffordshire, ST5 5BG, UK. 2. Department of Surgery & Department of Pharmacology & Physiology, Drexel University College of Medicine, Philadelphia, PA 19102, USA.
Abstract
AIM: To achieve high and sustained magnetic particle loading in a proliferative and endocytotically active neural transplant population (astrocytes) through tailored magnetite content in polymeric iron oxide particles. MATERIALS & METHODS: MPs of varying magnetite content were applied to primary-derived rat cortical astrocytes ± static/oscillating magnetic fields to assess labeling efficiency and safety. RESULTS: Higher magnetite content particles display high but safe accumulation in astrocytes, with longer-term label retention versus lower/no magnetite content particles. Magnetic fields enhanced loading extent. Dynamic live cell imaging of dividing labeled astrocytes demonstrated that particle distribution into daughter cells is predominantly 'asymmetric'. CONCLUSION: These findings could inform protocols to achieve efficient MP loading into neural transplant cells, with significant implications for post-transplantation tracking/localization.
AIM: To achieve high and sustained magnetic particle loading in a proliferative and endocytotically active neural transplant population (astrocytes) through tailored magnetite content in polymeric iron oxide particles. MATERIALS & METHODS: MPs of varying magnetite content were applied to primary-derived rat cortical astrocytes ± static/oscillating magnetic fields to assess labeling efficiency and safety. RESULTS: Higher magnetite content particles display high but safe accumulation in astrocytes, with longer-term label retention versus lower/no magnetite content particles. Magnetic fields enhanced loading extent. Dynamic live cell imaging of dividing labeled astrocytes demonstrated that particle distribution into daughter cells is predominantly 'asymmetric'. CONCLUSION: These findings could inform protocols to achieve efficient MP loading into neural transplant cells, with significant implications for post-transplantation tracking/localization.
Authors: Anat Yanai; Urs O Häfeli; Andrew L Metcalfe; Peter Soema; Lois Addo; Cheryl Y Gregory-Evans; Kelvin Po; Xianghong Shan; Orson L Moritz; Kevin Gregory-Evans Journal: Cell Transplant Date: 2012-03-08 Impact factor: 4.064
Authors: Christopher F Adams; Ahmad Rai; Gregor Sneddon; Humphrey H P Yiu; Boris Polyak; Divya M Chari Journal: Nanomedicine Date: 2014-07-17 Impact factor: 5.307