M Victoria1, L F L Tort1, A Lizasoain1, M García1, M Castells1, M Berois2, M Divizia3, J P G Leite4, M P Miagostovich4, J Cristina5, R Colina1. 1. Laboratory of Molecular Virology, Department of Biological Sciences, Regional Norte, CENUR Litoral Norte, Universidad de la República, Salto, Uruguay. 2. Virology Section, School of Sciences, Universidad de la República, Montevideo, Uruguay. 3. Laboratory of Environmental Virology, Department of Experimental Medicine and Surgery, Tor Vergata University, Rome, Italy. 4. Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. 5. Laboratory of Molecular Virology, Nuclear Investigation Center, School of Sciences, Universidad de la República, Montevideo, Uruguay.
Abstract
AIMS: To determine the prevalence and molecular epidemiology of norovirus (NoV) genogroup I (GI) and GII in Uruguay. METHODS AND RESULTS: One hundred and sixteen sewage samples were collected in six cities (Bella Unión, Salto, Paysandú, Fray Bentos, Melo and Treinta y Tres) from March 2011 to April 2013, viruses were concentrated by ultracentrifugation and NoV studies were performed by semi-nested RT-PCR (partial capsid region). NoV were detected in samples from all the cities and detected in 72% (84/116) of the samples with nine of them belonging to GI, 48 to GII and 27 to both genogroups. Remarkably, a high genetic diversity was identified: GII.2 (n = 13), GII.4 (n = 13), GI.1 (n = 5), GI.4 (n = 5), GI.8 (n = 4), GII.13 (n = 4), GII.1 (n = 3), GII.6 (n = 3), GI.3 (n = 1), GI.5 (n = 1), GI.6 (n = 1), GII.3 (n = 1), GII.17 (n = 1). Interestingly, a complete replacement of GII.4 New Orleans 2009 by GII.4 Sydney 2012 variants during 2012 was evidenced. CONCLUSION: This study reveals a high circulation of different NoV GI and GII genotypes in sewage evidencing a replacement of GII.4 variants. SIGNIFICANCE AND IMPACT OF STUDY: This approach can be used as an indicator of the presence of a new GII.4 variant which can originate an increase in acute gastroenteritis outbreaks worldwide.
AIMS: To determine the prevalence and molecular epidemiology of norovirus (NoV) genogroup I (GI) and GII in Uruguay. METHODS AND RESULTS: One hundred and sixteen sewage samples were collected in six cities (Bella Unión, Salto, Paysandú, Fray Bentos, Melo and Treinta y Tres) from March 2011 to April 2013, viruses were concentrated by ultracentrifugation and NoV studies were performed by semi-nested RT-PCR (partial capsid region). NoV were detected in samples from all the cities and detected in 72% (84/116) of the samples with nine of them belonging to GI, 48 to GII and 27 to both genogroups. Remarkably, a high genetic diversity was identified: GII.2 (n = 13), GII.4 (n = 13), GI.1 (n = 5), GI.4 (n = 5), GI.8 (n = 4), GII.13 (n = 4), GII.1 (n = 3), GII.6 (n = 3), GI.3 (n = 1), GI.5 (n = 1), GI.6 (n = 1), GII.3 (n = 1), GII.17 (n = 1). Interestingly, a complete replacement of GII.4 New Orleans 2009 by GII.4 Sydney 2012 variants during 2012 was evidenced. CONCLUSION: This study reveals a high circulation of different NoV GI and GII genotypes in sewage evidencing a replacement of GII.4 variants. SIGNIFICANCE AND IMPACT OF STUDY: This approach can be used as an indicator of the presence of a new GII.4 variant which can originate an increase in acute gastroenteritis outbreaks worldwide.
Authors: E Suffredini; M Iaconelli; M Equestre; B Valdazo-González; A R Ciccaglione; C Marcantonio; S Della Libera; F Bignami; G La Rosa Journal: Food Environ Virol Date: 2017-11-28 Impact factor: 2.778
Authors: P Gamazo; M Victoria; J F Schijven; E Alvareda; L F L Tort; J Ramos; L A Lizasoain; G Sapriza; M Castells; L Bessone; R Colina Journal: Food Environ Virol Date: 2019-11-12 Impact factor: 2.778
Authors: G La Rosa; S Della Libera; M Iaconelli; Y T R Proroga; D De Medici; V Martella; E Suffredini Journal: Food Environ Virol Date: 2017-03-03 Impact factor: 2.778
Authors: Andrés Lizasoain; Fernanda M Burlandy; Matías Victoria; Luis F López Tort; Edson E da Silva; Rodney Colina Journal: Food Environ Virol Date: 2018-06-16 Impact factor: 2.778
Authors: Jennifer H Lun; Joanne Hewitt; Alefiya Sitabkhan; John-Sebastian Eden; Daniel Enosi Tuipulotu; Natalie E Netzler; Leigh Morrell; Juan Merif; Richard Jones; Bixing Huang; David Warrilow; Kelly-Anne Ressler; Mark J Ferson; Dominic E Dwyer; Jen Kok; William D Rawlinson; Daniel Deere; Nicholas D Crosbie; Peter A White Journal: Emerg Microbes Infect Date: 2018-03-29 Impact factor: 7.163