Erratum to: MetCap: A bioinformatics probe design pipeline for large-scale targeted metagenomics.

Erratum

In our article [1], the numbers of probes and sequences for the CAZy dataset in the Additional file 3, table S3 (A) (Table 1 here) have been switched. The corrected table S3 (A) is presented in this erratum.

Table 1

(A): Family-wise summary of generated probes based on sequences downloaded from CAZy database

Family	Archaea	Bacteria	Eukaryota	Unclassified	Virus	Total probes	Total sequences
CBM	439	26910	7424	134	453	35360	23302
CE	311	14646	3254	66	9	18286	13787
GH	2754	97488	33643	1457	7382	142724	110923
GT	7755	106687	30802	160	683	146087	103952
PL	57	4663	1082	6	51	5859	6580
Total	11316	250394	76205	1823	8578	348316	258544

In the following three parts of our article [1] we would like to correct the numbers for probes and sequences accordingly.

Abstract (Results), page 1

To illustrate the advantage of a targeted metagenome approach, we have generated more than 400,000 probes that match more than 300,000 publicly available sequences related to carbon degradation, and used these probes for targeted sequencing in a soil metagenome study.

Results and discussion, page 6

In this study, 306,525 nucleotide sequences were extracted through the pipeline and used as a proof of concept. A list of group-wise collected sequences and generated probes from databases are given as Additional file 3: Table S3. In total, 406,277 unique probes were produced from these extracted nucleotide sequences in this study with the following criteria: length (50mer), GC contents (35-65), melting temperature (55-65), and 3 probes per cluster on 90 % cluster similarity.

Results and discussion, page 8

Among the downloaded sequences (Additional file 3: Table S3), 258,544 sequences belong to the CAZy database from four major families: Glycoside Hydrolase (110,923), Glycosyl Transferases (103,952), Carbohydrate Esterases (13,787), Polysaccharide Lyases (6,580), and an associated module, Carbohydrate binding-modules (23,302) and 348,316 probes have been designed from these sequences.