X Qi1, J Han2, P Zhao3, X Dong4, S Gong1. 1. Department of Medicine, Qingdao University, No. 16, Ningde Road, Shinan District, QingDao, SanDong Shandong, China. 2. Public Security Bureau, No. 128, Fuzhou Road, Shibei District, QingDao, SanDong Qingdao, Shandong, China. 3. Department of Pathology, Affiliated Hospital of Qiingdao University, No. 59, Haier Road, Laoshan District, QingDao, Shandong, China. pengzhao2015@sina.com. 4. Department of Pathology, Affiliated Hospital of Qiingdao University, No. 59, Haier Road, Laoshan District, QingDao, Shandong, China.
Abstract
OBJECTIVE: The purpose of this work is to investigate the expression of S100A4 and P53 and their correlation with myocardial collagen fibers in hypertrophic cardiomyopathy (HCM). PATIENTS AND METHODS: Myocardial tissue obtained from 10 patients undergoing HCM surgery (HCM group) was compared to myocardial tissue obtained from 10 traffic accident deaths (healthy control group). Collagen volume fractions were visualized and compared using sirius red F3B(SR) staining. Immunohistochemistry S-P and in situ hybridization techniques were used to test the different expressions of S100A4, P53 proteins, and their mRNA. Imaging and statistical methods were used for quantitative analysis. RESULTS: The collagen volume fraction in the HCM group was significantly higher than that in the control group. The integral optical density values of S100A4 and P53 in cardiomyocytes of the HCM group were also significantly higher than in the control group. CONCLUSION: The increased content of collagen fibers and overexpression of S100A4 and P53 may play an important role in myocardial fibrosis of HCM, and they can be used in future HCM research. Blockade of S100A4 may have therapeutic potential in HCM by attenuating cardiac fibrosis.
OBJECTIVE: The purpose of this work is to investigate the expression of S100A4 and P53 and their correlation with myocardial collagen fibers in hypertrophic cardiomyopathy (HCM). PATIENTS AND METHODS: Myocardial tissue obtained from 10 patients undergoing HCM surgery (HCM group) was compared to myocardial tissue obtained from 10 traffic accident deaths (healthy control group). Collagen volume fractions were visualized and compared using sirius red F3B(SR) staining. Immunohistochemistry S-P and in situ hybridization techniques were used to test the different expressions of S100A4, P53 proteins, and their mRNA. Imaging and statistical methods were used for quantitative analysis. RESULTS: The collagen volume fraction in the HCM group was significantly higher than that in the control group. The integral optical density values of S100A4 and P53 in cardiomyocytes of the HCM group were also significantly higher than in the control group. CONCLUSION: The increased content of collagen fibers and overexpression of S100A4 and P53 may play an important role in myocardial fibrosis of HCM, and they can be used in future HCM research. Blockade of S100A4 may have therapeutic potential in HCM by attenuating cardiac fibrosis.
Entities:
Keywords:
Arrhythmia, cardiac; Biomarkers; Cardiac hypertrophy; Fibrosis; Sudden cardiac death
Authors: M Grigorian; S Andresen; E Tulchinsky; M Kriajevska; C Carlberg; C Kruse; M Cohn; N Ambartsumian; A Christensen; G Selivanova; E Lukanidin Journal: J Biol Chem Date: 2001-03-05 Impact factor: 5.157
Authors: Maria Rosario Fernandez-Fernandez; Dmitry B Veprintsev; Alan R Fersht Journal: Proc Natl Acad Sci U S A Date: 2005-03-21 Impact factor: 11.205
Authors: D Cesselli; I Jakoniuk; L Barlucchi; A P Beltrami; T H Hintze; B Nadal-Ginard; J Kajstura; A Leri; P Anversa Journal: Circ Res Date: 2001-08-03 Impact factor: 17.367
Authors: Mikael Schneider; Sawa Kostin; Claes C Strøm; Mark Aplin; Stig Lyngbaek; Juliane Theilade; Mariam Grigorian; Claus B Andersen; Eugene Lukanidin; Jakob Lerche Hansen; Søren P Sheikh Journal: Cardiovasc Res Date: 2007-04-06 Impact factor: 10.787
Authors: Simon Braumann; Tilo Thottakara; Sabrina Stücker; Silke Reischmann-Düsener; Elisabeth Krämer; Julia Groß; Marc N Hirt; Shirin Doroudgar; Lucie Carrier; Felix W Friedrich Journal: Front Physiol Date: 2018-09-19 Impact factor: 4.566
Authors: Maria Laggner; Philipp Hacker; Felicitas Oberndorfer; Jonas Bauer; Thomas Raunegger; Christian Gerges; Tamás Szerafin; Jürgen Thanner; Irene Lang; Nika Skoro-Sajer; Hendrik Jan Ankersmit; Bernhard Moser Journal: Biology (Basel) Date: 2022-01-12