Chao Shang1, Yan Guo2, Hui Zhang3, Yi-Xue Xue4,5. 1. Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang, 110122, China. 2. Department of Central Laboratory, School of Stomatology, China Medical University, Shenyang, 110007, China. 3. Department of Urinary Surgery, Shengjing Hospital, China Medical University, Shenyang, 110004, China. 4. Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang, 110122, China. xueyixue888@163.com. 5. Department of Neurobiology, China Medical University, No. 77 Puhe Road, Shenyang North New Area, Shenyang, 110122, China. xueyixue888@163.com.
Abstract
PURPOSE: In this study, whether HOTAIR is a prognostic biomarker will be detected, and its regulative effects of chemosensitivity to doxorubicin in TCC cells will be examined. METHODS: The expression of HOTAIR was detected by quantitative real-time PCR. Overall survival rate was calculated by Kaplan-Meier method with the log-rank test for comparisons. MTT assay was used to detect cell proliferation ability and chemosensitivity. Dual-color flow cytometric method was used to detect cell apoptosis. RESULTS: HOTAIR was up-regulated in bladder transitional cell carcinoma (TCC) tissues and cell lines compared with normal bladder transitional cell (NBTC) tissues and bladder epithelial immortalized SV-HUC-1 cells, and its expression level had positive correlation with histological grades of TCC. Moreover, HOTAIR was an independent prognostic biomarker of overall survival for TCC patients. The expression and silence vector for HOTAIR were transfected into T24 and J82 cells to up-regulate and silence the HOTAIR expression, respectively. In T24 and J82 cells, HOTAIR over-expression promoted cell proliferation and inhibited chemosensitivity to doxorubicin and cell apoptosis induced by doxorubicin; silence of HOTAIR showed opposite regulative effects. CONCLUSIONS: In summary, lncRNA HOTAIR was an independent prognostic biomarker of overall survival in TCC patients and could regulate chemosensitivity to doxorubicin of human TCC cells. HOTAIR might provide a new potential therapeutic target and stratagem for TCC.
PURPOSE: In this study, whether HOTAIR is a prognostic biomarker will be detected, and its regulative effects of chemosensitivity to doxorubicin in TCC cells will be examined. METHODS: The expression of HOTAIR was detected by quantitative real-time PCR. Overall survival rate was calculated by Kaplan-Meier method with the log-rank test for comparisons. MTT assay was used to detect cell proliferation ability and chemosensitivity. Dual-color flow cytometric method was used to detect cell apoptosis. RESULTS:HOTAIR was up-regulated in bladder transitional cell carcinoma (TCC) tissues and cell lines compared with normal bladder transitional cell (NBTC) tissues and bladder epithelial immortalized SV-HUC-1 cells, and its expression level had positive correlation with histological grades of TCC. Moreover, HOTAIR was an independent prognostic biomarker of overall survival for TCC patients. The expression and silence vector for HOTAIR were transfected into T24 and J82 cells to up-regulate and silence the HOTAIR expression, respectively. In T24 and J82 cells, HOTAIR over-expression promoted cell proliferation and inhibited chemosensitivity to doxorubicin and cell apoptosis induced by doxorubicin; silence of HOTAIR showed opposite regulative effects. CONCLUSIONS: In summary, lncRNA HOTAIR was an independent prognostic biomarker of overall survival in TCC patients and could regulate chemosensitivity to doxorubicin of human TCC cells. HOTAIR might provide a new potential therapeutic target and stratagem for TCC.
Authors: David Padua; Swapna Mahurkar-Joshi; Ivy Ka Man Law; Christos Polytarchou; John P Vu; Joseph R Pisegna; David Shih; Dimitrios Iliopoulos; Charalabos Pothoulakis Journal: Am J Physiol Gastrointest Liver Physiol Date: 2016-08-04 Impact factor: 4.052
Authors: Lei Hu; Shu-Hui Chen; Qiao-Li Lv; Bao Sun; Qiang Qu; Chong-Zhen Qin; Lan Fan; Ying Guo; Lin Cheng; Hong-Hao Zhou Journal: Int J Environ Res Public Health Date: 2016-05-30 Impact factor: 3.390