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Literature DB >> 26780499

DNA Electroporation, Isolation and Imaging of Myofibers.

Alexis R Demonbreun¹, Elizabeth M McNally².

Abstract

Mature muscle has a unique structure that is amenable to live cell imaging. Herein, we describe the experimental protocol for expressing fluorescently labeled proteins in the flexor digitorum brevis (FDB) muscle. Conditions have been optimized to provide a large number of high quality myofibers expressing the electroporated plasmid while minimizing muscle damage. The method employs fluorescent tags on various proteins. Combining this expression method with high resolution confocal microscopy permits live cell imaging, including imaging after laser-induced damage. Fluorescent dyes combined with imaging of fluorescently-tagged proteins provides information regarding the basic structure of muscle and its response to stimuli.

Entities: CellLine Chemical Disease Gene Mutation Species

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Year: 2015 PMID： 26780499 PMCID： PMC4758766 DOI： 10.3791/53551

Source DB: PubMed Journal: J Vis Exp ISSN： 1940-087X Impact factor: 1.355

16 in total

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Authors: Kayleigh A Swaggart; Alexis R Demonbreun; Andy H Vo; Kaitlin E Swanson; Ellis Y Kim; John P Fahrenbach; Jenan Holley-Cuthrell; Ascia Eskin; Zugen Chen; Kevin Squire; Ahlke Heydemann; Abraham A Palmer; Stanley F Nelson; Elizabeth M McNally
Journal: Proc Natl Acad Sci U S A Date: 2014-04-09 Impact factor: 11.205

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Authors: Chuanxi Cai; Haruko Masumiya; Noah Weisleder; Noriyuki Matsuda; Miyuki Nishi; Moonsun Hwang; Jae-Kyun Ko; Peihui Lin; Angela Thornton; Xiaoli Zhao; Zui Pan; Shinji Komazaki; Marco Brotto; Hiroshi Takeshima; Jianjie Ma
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9. DNA transfection of mammalian skeletal muscles using in vivo electroporation.

Authors: Marino DiFranco; Marbella Quinonez; Joana Capote; Julio Vergara
Journal: J Vis Exp Date: 2009-10-19 Impact factor: 1.355

10. Immortalized pathological human myoblasts: towards a universal tool for the study of neuromuscular disorders.

Authors: Kamel Mamchaoui; Capucine Trollet; Anne Bigot; Elisa Negroni; Soraya Chaouch; Annie Wolff; Prashanth K Kandalla; Solenne Marie; James Di Santo; Jean Lacau St Guily; Francesco Muntoni; Jihee Kim; Susanne Philippi; Simone Spuler; Nicolas Levy; Sergiu C Blumen; Thomas Voit; Woodring E Wright; Ahmed Aamiri; Gillian Butler-Browne; Vincent Mouly
Journal: Skelet Muscle Date: 2011-11-01 Impact factor: 4.912

16 in total

1. Intermittent Glucocorticoid Dosing Improves Muscle Repair and Function in Mice with Limb-Girdle Muscular Dystrophy.

Authors: Mattia Quattrocelli; Isabella M Salamone; Patrick G Page; James L Warner; Alexis R Demonbreun; Elizabeth M McNally
Journal: Am J Pathol Date: 2017-08-18 Impact factor: 4.307

2. Intermittent glucocorticoid steroid dosing enhances muscle repair without eliciting muscle atrophy.

Authors: Mattia Quattrocelli; David Y Barefield; James L Warner; Andy H Vo; Michele Hadhazy; Judy U Earley; Alexis R Demonbreun; Elizabeth M McNally
Journal: J Clin Invest Date: 2017-05-08 Impact factor: 14.808

3. Pulsed glucocorticoids enhance dystrophic muscle performance through epigenetic-metabolic reprogramming.

Authors: Mattia Quattrocelli; Aaron S Zelikovich; Zhen Jiang; Clara Bien Peek; Alexis R Demonbreun; Nancy L Kuntz; Grant D Barish; Saptarsi M Haldar; Joseph Bass; Elizabeth M McNally
Journal: JCI Insight Date: 2019-12-19

4. Recombinant annexin A6 promotes membrane repair and protects against muscle injury.

Authors: Alexis R Demonbreun; Katherine S Fallon; Claire C Oosterbaan; Elena Bogdanovic; James L Warner; Jordan J Sell; Patrick G Page; Mattia Quattrocelli; David Y Barefield; Elizabeth M McNally
Journal: J Clin Invest Date: 2019-11-01 Impact factor: 14.808

5. Enhanced Muscular Dystrophy from Loss of Dysferlin Is Accompanied by Impaired Annexin A6 Translocation after Sarcolemmal Disruption.

Authors: Alexis R Demonbreun; Madison V Allen; James L Warner; David Y Barefield; Swathi Krishnan; Kaitlin E Swanson; Judy U Earley; Elizabeth M McNally
Journal: Am J Pathol Date: 2016-04-09 Impact factor: 4.307