Literature DB >> 26779654

High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis.

Kim V Berghaus1, Seok H Yun2, Giuliano Scarcelli3.   

Abstract

The goal of this protocol is to build a parallel high-extinction and high-resolution optical Brillouin spectrometer. Brillouin spectroscopy is a non-contact measurement method that can be used to obtain direct readouts of viscoelastic material properties. It has been a useful tool in material characterization, structural monitoring and environmental sensing. In the past, Brillouin spectroscopy has usually employed scanning Fabry-Perot etalons to perform spectral analysis. This process requires high illumination power and long acquisition times, making the technique unsuitable for biomedical applications. A recently introduced novel spectrometer overcomes this challenge by employing two VIPAs in a cross-axis configuration. This innovation enables sub-Gigahertz (GHz) resolution spectral analysis with sub-second acquisition time and illumination power within the safety limits of biological tissue. The multiple new applications facilitated by this improvement are currently being explored in biological research and clinical application.

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Mesh:

Year:  2015        PMID: 26779654      PMCID: PMC4758767          DOI: 10.3791/53468

Source DB:  PubMed          Journal:  J Vis Exp        ISSN: 1940-087X            Impact factor:   1.355


  10 in total

1.  Video-rate scanning confocal microscopy and microendoscopy.

Authors:  Alexander J Nichols; Conor L Evans
Journal:  J Vis Exp       Date:  2011-10-20       Impact factor: 1.355

2.  In vivo measurement of age-related stiffening in the crystalline lens by Brillouin optical microscopy.

Authors:  Giuliano Scarcelli; Pilhan Kim; Seok Hyun Yun
Journal:  Biophys J       Date:  2011-09-20       Impact factor: 4.033

3.  Confocal Brillouin microscopy for three-dimensional mechanical imaging.

Authors:  Giuliano Scarcelli; Seok Hyun Yun
Journal:  Nat Photonics       Date:  2007-12-09       Impact factor: 38.771

4.  Brillouin microscopy of collagen crosslinking: noncontact depth-dependent analysis of corneal elastic modulus.

Authors:  Giuliano Scarcelli; Sabine Kling; Elena Quijano; Roberto Pineda; Susana Marcos; Seok Hyun Yun
Journal:  Invest Ophthalmol Vis Sci       Date:  2013-02-19       Impact factor: 4.799

5.  Background clean-up in Brillouin microspectroscopy of scattering medium.

Authors:  Zhaokai Meng; Andrew J Traverso; Vladislav V Yakovlev
Journal:  Opt Express       Date:  2014-03-10       Impact factor: 3.894

6.  Brillouin spectroscopy as a new method of screening for increased CSF total protein during bacterial meningitis.

Authors:  Zachary Steelman; Zhaokai Meng; Andrew J Traverso; Vladislav V Yakovlev
Journal:  J Biophotonics       Date:  2014-07-15       Impact factor: 3.207

7.  In vivo biomechanical mapping of normal and keratoconus corneas.

Authors:  Giuliano Scarcelli; Sebastien Besner; Roberto Pineda; Patricia Kalout; Seok Hyun Yun
Journal:  JAMA Ophthalmol       Date:  2015-04       Impact factor: 7.389

8.  Multistage VIPA etalons for high-extinction parallel Brillouin spectroscopy.

Authors:  Giuliano Scarcelli; Seok Hyun Yun
Journal:  Opt Express       Date:  2011-05-23       Impact factor: 3.894

9.  In vivo Brillouin optical microscopy of the human eye.

Authors:  Giuliano Scarcelli; Seok Hyun Yun
Journal:  Opt Express       Date:  2012-04-09       Impact factor: 3.894

10.  Spatially resolved Brillouin spectroscopy to determine the rheological properties of the eye lens.

Authors:  Stephan Reiß; Gerolf Burau; Oliver Stachs; Rudolf Guthoff; Heinrich Stolz
Journal:  Biomed Opt Express       Date:  2011-07-05       Impact factor: 3.732

  10 in total
  16 in total

1.  Direct Three-Dimensional Measurement of Refractive Index via Dual Photon-Phonon Scattering.

Authors:  Antonio Fiore; Carlo Bevilacqua; Giuliano Scarcelli
Journal:  Phys Rev Lett       Date:  2019-03-15       Impact factor: 9.161

2.  Single etalon design for two-stage cross-axis VIPA spectroscopy.

Authors:  Antonio Fiore; Giuliano Scarcelli
Journal:  Biomed Opt Express       Date:  2019-02-28       Impact factor: 3.732

3.  Long-term Brillouin imaging of live cells with reduced absorption-mediated damage at 660 nm wavelength.

Authors:  Miloš Nikolić; Giuliano Scarcelli
Journal:  Biomed Opt Express       Date:  2019-03-04       Impact factor: 3.732

4.  Mechanical outcome of accelerated corneal crosslinking evaluated by Brillouin microscopy.

Authors:  Joshua N Webb; Johnny P Su; Giuliano Scarcelli
Journal:  J Cataract Refract Surg       Date:  2017-11       Impact factor: 3.351

5.  Brillouin flow cytometry for label-free mechanical phenotyping of the nucleus.

Authors:  Jitao Zhang; Xuefei A Nou; Hanyoup Kim; Giuliano Scarcelli
Journal:  Lab Chip       Date:  2017-02-14       Impact factor: 6.799

6.  Biomechanical Impact of Localized Corneal Cross-linking Beyond the Irradiated Treatment Area.

Authors:  Joshua N Webb; Erin Langille; Farhad Hafezi; J Bradley Randleman; Giuliano Scarcelli
Journal:  J Refract Surg       Date:  2019-04-01       Impact factor: 3.573

7.  Correlation of biomechanics and cancer cell phenotype by combined Brillouin and Raman spectroscopy of U87-MG glioblastoma cells.

Authors:  Jan Rix; Ortrud Uckermann; Katrin Kirsche; Gabriele Schackert; Edmund Koch; Matthias Kirsch; Roberta Galli
Journal:  J R Soc Interface       Date:  2022-07-13       Impact factor: 4.293

8.  Depth-Dependent Reduction of Biomechanical Efficacy of Contact Lens-Assisted Corneal Cross-linking Analyzed by Brillouin Microscopy.

Authors:  Hongyuan Zhang; Mehdi Roozbahani; Andre L Piccinini; Oren Golan; Farhad Hafezi; Giuliano Scarcelli; J Bradley Randleman
Journal:  J Refract Surg       Date:  2019-11-01       Impact factor: 3.573

9.  The role of cell body density in ruminant retina mechanics assessed by atomic force and Brillouin microscopy.

Authors:  Isabell P Weber; Seok Hyun Yun; Giuliano Scarcelli; Kristian Franze
Journal:  Phys Biol       Date:  2017-11-16       Impact factor: 2.583

10.  Multipass etalon cascade for high-resolution parallel spectroscopy.

Authors:  Antonio Fiore; Giuliano Scarcelli
Journal:  Opt Lett       Date:  2021-02-15       Impact factor: 3.776

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