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Literature DB >> 26778553

Purification and Structural Analysis of SUN and KASH Domain Proteins.

F Esra Demircioglu¹, Victor E Cruz¹, Thomas U Schwartz².

Abstract

Molecular tethers span the nuclear envelope to mechanically connect the cytoskeleton and nucleoskeleton. These bridge-like tethers, termed linkers of nucleoskeleton and cytoskeleton (LINC) complexes, consist of SUN proteins at the inner nuclear membrane and KASH proteins at the outer nuclear membrane. LINC complexes are central to a variety of cell activities including nuclear positioning and mechanotransduction, and LINC-related abnormalities are associated with a spectrum of tissue-specific diseases, termed laminopathies or envelopathies. Protocols used to study the biochemical and structural characteristics of core elements of SUN-KASH complexes are described here to facilitate further studies in this new field of cell biology.

Entities: Chemical Disease Gene Species

Keywords: KASH peptide; LINC complex; Nesprin; Nuclear envelope; SUN domain; SUN protein

Mesh：

Substances：

Year: 2015 PMID： 26778553 PMCID： PMC5544124 DOI： 10.1016/bs.mie.2015.08.011

Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600

35 in total

1. Structure of Sad1-UNC84 homology (SUN) domain defines features of molecular bridge in nuclear envelope.

Authors: Zhaocai Zhou; Xiulian Du; Zheng Cai; Xiaomin Song; Hongtao Zhang; Takako Mizuno; Emi Suzuki; Marla Rosanne Yee; Alan Berezov; Ramachandran Murali; Shiaw-Lin Wu; Barry L Karger; Mark I Greene; Qiang Wang
Journal: J Biol Chem Date: 2011-12-14 Impact factor: 5.157

Review 2. Nuclear lamins and laminopathies.

Authors: Howard J Worman
Journal: J Pathol Date: 2011-11-14 Impact factor: 7.996

Review 3. Interactions between nuclei and the cytoskeleton are mediated by SUN-KASH nuclear-envelope bridges.

Authors: Daniel A Starr; Heidi N Fridolfsson
Journal: Annu Rev Cell Dev Biol Date: 2010 Impact factor: 13.827

Review 4. Nuclei take a position: managing nuclear location.

Authors: Brian Burke; Kyle J Roux
Journal: Dev Cell Date: 2009-11 Impact factor: 12.270

5. Localized products of futile cycle/lrmp promote centrosome-nucleus attachment in the zebrafish zygote.

Authors: Robin E Lindeman; Francisco Pelegri
Journal: Curr Biol Date: 2012-04-26 Impact factor: 10.834

6. Nesprin interchain associations control nuclear size.

Authors: Wenshu Lu; Maria Schneider; Sascha Neumann; Verena-Maren Jaeger; Surayya Taranum; Martina Munck; Sarah Cartwright; Christine Richardson; James Carthew; Kowoon Noh; Martin Goldberg; Angelika A Noegel; Iakowos Karakesisoglou
Journal: Cell Mol Life Sci Date: 2012-06-01 Impact factor: 9.261

7. Role of ANC-1 in tethering nuclei to the actin cytoskeleton.

Authors: Daniel A Starr; Min Han
Journal: Science Date: 2002-08-08 Impact factor: 47.728

Review 8. KASHing up with the nucleus: novel functional roles of KASH proteins at the cytoplasmic surface of the nucleus.

Authors: G W Gant Luxton; Daniel A Starr
Journal: Curr Opin Cell Biol Date: 2014-04-03 Impact factor: 8.382

Purification and Structural Analysis of SUN and KASH Domain Proteins.

1. Structure of Sad1-UNC84 homology (SUN) domain defines features of molecular bridge in nuclear envelope.

Review 2. Nuclear lamins and laminopathies.

Review 3. Interactions between nuclei and the cytoskeleton are mediated by SUN-KASH nuclear-envelope bridges.

Review 4. Nuclei take a position: managing nuclear location.

5. Localized products of futile cycle/lrmp promote centrosome-nucleus attachment in the zebrafish zygote.

6. Nesprin interchain associations control nuclear size.

7. Role of ANC-1 in tethering nuclei to the actin cytoskeleton.

Review 8. KASHing up with the nucleus: novel functional roles of KASH proteins at the cytoplasmic surface of the nucleus.

Review 9. Accessorizing and anchoring the LINC complex for multifunctionality.

10. A mammalian KASH domain protein coupling meiotic chromosomes to the cytoskeleton.

Review 1. Mechanotransduction in tumor progression: The dark side of the force.

2. LINC complexes promote homologous recombination in part through inhibition of nonhomologous end joining.

Review 3. A Glance at the Nuclear Envelope Spectrin Repeat Protein 3.

4. A molecular mechanism for LINC complex branching by structurally diverse SUN-KASH 6:6 assemblies.