| Literature DB >> 26776044 |
Yan Zhang1, Jieqiong Zhang2, Wei Sheng3, Shuo Wang3, Tong-Jen Fu4.
Abstract
The effects of dry and moist heat, autoclave sterilization and high-pressure treatment on the biochemical characteristics and immunological properties of almond proteins were investigated. Changes in the solubility and immunoreactivity of almond proteins extracted from treated almond flour were evaluated using a total protein assay, indirect competitive inhibition enzyme-linked immunosorbent assay (IC-ELISA), and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Almond proteins were stable during dry-heat treatment at temperatures below 250°C. Dry heat at 400°C, boiling, autoclave sterilization and high-pressure treatment in the presence of water at ⩾ 500 MPa greatly reduced the solubility and immunoreactivity of almond proteins. SDS-PAGE revealed that the protein profiles of almond flour samples treated under these conditions also changed significantly. The synergistic effects of heat, pressure and the presence of water contributed to significant changes in solubility and immunoreactivity of almond proteins.Entities:
Keywords: Almond protein; Citric acid (PubChem CID:311); DMSO (PubChem CID:679); Glycine (PubChem CID:750); Heat treatment; High pressure; Immunoreactivity; SDS (PubChem CID:3423265); Sodium carbonate (PubChem CID:10340); Solubility; Tris (hydroxymethyl) aminomethane (Tris) (PubChem CID:176250); Tween 20 (PubChem CID:443314)
Mesh：
Year: 2015 PMID： 26776044 DOI： 10.1016/j.foodchem.2015.12.063
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514