M Bachy1, I Sherifi2, F Zadegan3, H Petite4, R Vialle5, D Hannouche3. 1. Laboratoire de bioingénierie et bioimagerie ostéo-articulaire (B2OA), CNRS UMR 7052, université Denis-Diderot Paris VII, Paris, France; Université Pierre et Marie Curie Paris 6, Department of Pediatric Orthopaedics, Armand-Trousseau Hospital, 26, avenue du Dr-Arnold-Netter, 75571 Paris cedex 12, France; The MAMUTH Hospital-University Department for Innovative Therapies in Musculoskeletal Diseases, Armand-Trousseau Hospital, 26, avenue du Docteur-Arnold-Netter, 75571 Paris cedex 12, France. 2. Laboratoire de bioingénierie et bioimagerie ostéo-articulaire (B2OA), CNRS UMR 7052, université Denis-Diderot Paris VII, Paris, France; The Mount Sinai Hospital One Gustave L.-Levy Place, New York, USA. 3. Laboratoire de bioingénierie et bioimagerie ostéo-articulaire (B2OA), CNRS UMR 7052, université Denis-Diderot Paris VII, Paris, France; Service de chirurgie orthopédique et réparatrice, hôpital Lariboisière, AP-HP, université Denis-Diderot Paris VII, Paris, France. 4. Laboratoire de bioingénierie et bioimagerie ostéo-articulaire (B2OA), CNRS UMR 7052, université Denis-Diderot Paris VII, Paris, France. 5. Université Pierre et Marie Curie Paris 6, Department of Pediatric Orthopaedics, Armand-Trousseau Hospital, 26, avenue du Dr-Arnold-Netter, 75571 Paris cedex 12, France; The MAMUTH Hospital-University Department for Innovative Therapies in Musculoskeletal Diseases, Armand-Trousseau Hospital, 26, avenue du Docteur-Arnold-Netter, 75571 Paris cedex 12, France. Electronic address: raphael.vialle@trs.aphp.fr.
Abstract
BACKGROUND: Tissue engineering strategies include both cell-based and cell homing therapies. Ligamentous tissues are highly specialized and constitute vital components of the musculoskeletal system. Their damage causes significant morbidity and loss in function. HYPOTHESIS: The aim of this study is to analyze tendinous graft integration, cell repopulation and ligamentization by using GFP+/- allografts in GFP+/- transgenic New Zealand white (NZW) rabbits. MATERIAL AND METHODS: Graft implantation was designed to closely mimic anterior cruciate ligament (ACL) repair surgery. Allografts were implanted in 8 NZW rabbits and assessed at 5 days, 3 weeks and 6 weeks through: (1) arthroCT imaging, (2) morphological analysis of the transplanted allograft, (3) histological analysis, (4) collagen type I immunochemistry, and (5) GFP cell tracking. Collagen remodeling was appreciated at 3 and 6 weeks. RESULTS: Graft repopulation with host cells, chondrocyte-like cells at the tendon-bone interface and graft corticalization in the bone tunnels were noticed at 3 weeks. By contrast we noticed a central necrosis aspect in the allografts intra-articularly at 6 weeks with a cell migration towards the graft edge near the synovium. DISCUSSION: Our study has served to gain a better understanding of tendinous allograft bone integration, ligamentization and allograft repopulation. We believe that both cell-based therapies and cell homing therapies are beneficial in ligament tissue engineering. Future studies may elucidate whether cell repopulation occurs with pre-differentiated or progenitor cells. We believe that both cell-based therapies and cell homing therapies are beneficial in ligament tissue engineering. LEVEL OF EVIDENCE: Level V (animal study).
BACKGROUND: Tissue engineering strategies include both cell-based and cell homing therapies. Ligamentous tissues are highly specialized and constitute vital components of the musculoskeletal system. Their damage causes significant morbidity and loss in function. HYPOTHESIS: The aim of this study is to analyze tendinous graft integration, cell repopulation and ligamentization by using GFP+/- allografts in GFP+/- transgenic New Zealand white (NZW) rabbits. MATERIAL AND METHODS: Graft implantation was designed to closely mimic anterior cruciate ligament (ACL) repair surgery. Allografts were implanted in 8 NZW rabbits and assessed at 5 days, 3 weeks and 6 weeks through: (1) arthroCT imaging, (2) morphological analysis of the transplanted allograft, (3) histological analysis, (4) collagen type I immunochemistry, and (5) GFP cell tracking. Collagen remodeling was appreciated at 3 and 6 weeks. RESULTS: Graft repopulation with host cells, chondrocyte-like cells at the tendon-bone interface and graft corticalization in the bone tunnels were noticed at 3 weeks. By contrast we noticed a central necrosis aspect in the allografts intra-articularly at 6 weeks with a cell migration towards the graft edge near the synovium. DISCUSSION: Our study has served to gain a better understanding of tendinous allograft bone integration, ligamentization and allograft repopulation. We believe that both cell-based therapies and cell homing therapies are beneficial in ligament tissue engineering. Future studies may elucidate whether cell repopulation occurs with pre-differentiated or progenitor cells. We believe that both cell-based therapies and cell homing therapies are beneficial in ligament tissue engineering. LEVEL OF EVIDENCE: Level V (animal study).
Authors: Timur B Kamalitdinov; Keitaro Fujino; Snehal S Shetye; Xi Jiang; Yaping Ye; Ashley B Rodriguez; Andrew F Kuntz; Miltiadis H Zgonis; Nathaniel A Dyment Journal: J Orthop Res Date: 2019-07-11 Impact factor: 3.494
Authors: Yusuke Hagiwara; Felix Dyrna; Andrew F Kuntz; Douglas J Adams; Nathaniel A Dyment Journal: Ann N Y Acad Sci Date: 2019-10-09 Impact factor: 5.691