Viviane Hass1, Issis Luque-Martinez2, Miguel Angel Muñoz3, Mario Felipe Gutierrez Reyes4, Gabriel Abuna5, Mario Alexandre Coelho Sinhoreti5, Alex Yi Liu6, Alessandro D Loguercio7, Yong Wang6, Alessandra Reis8. 1. Department of Restorative Dentistry, School of Dentistry, State University of Ponta Grossa, Paraná, Brazil; Department of Oral & Craniofacial Sciences, University of Missouri Kansas City, Kansas City, MO, USA. 2. Dentistry Academic Unit, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile. 3. Department of Biomaterials, School of Dentistry, Universidad Val Paraíso, Valparaíso, Chile. 4. Department of Restorative Dentistry, School of Dentistry, State University of Ponta Grossa, Paraná, Brazil; Institute for Research in Dental Sciences, Faculty of Odontology, University of Chile, Chile. 5. Department of Dental Materials, Piracicaba Dental School, Piracicaba, São Paulo, Brazil. 6. Department of Oral & Craniofacial Sciences, University of Missouri Kansas City, Kansas City, MO, USA. 7. Department of Restorative Dentistry, School of Dentistry, State University of Ponta Grossa, Paraná, Brazil. 8. Department of Restorative Dentistry, School of Dentistry, State University of Ponta Grossa, Paraná, Brazil. Electronic address: reis_ale@hotmail.com.
Abstract
OBJECTIVES: This study evaluated the effect of etching using 2% proanthocynidin-containing 10% phosphoric acid 2% PA/10% PhA vs. 35% phosphoric acid 35% PhA on immediate (IM) and 6-months (6M) resin-enamel microshear bond strength (μSBS), resin-dentin microtensile bond strength (μTBS), nanoleakage (NL) and as well as in situ MMP inhibition potential. METHODS: The dentin surface of human were exposed and then etched using 35% phosphoric acid for 15s or 2% PA/10% phosphoric acid for 30s. After rinsing with water, the dentin was bonded with Single Bond Plus (3M ESPE) and composite build-ups were constructed, followed by polymerization. The teeth were sectioned and the bonds were testing for microtensile bond strength (μTBS) and by SEM for NL analysis at IM and 6M. For MMP activity, resin-dentin slices were prepared for in situ zymography, and analyzed under confocal microscopy. For μSBS, others teeth had flattened enamel surfaces etched according the experimental groups and prepared to microshear procedure. The specimens were tested IM and after 6M by microshear bond strength. The data were submitted to two-way repeated measures ANOVA and Tukey's test (α=0.05). RESULTS: Acid-etching using the 2% PA/10% phosphoric acid did not lower the μTBS in IM (p>0.05) compared to the control 35% phosphoric acid group. However, after 6M, only the 2% PA/10% PhA etched dentin had remained stable the resin-dentin bond strength (p<0.05). Bonds made with 35% PhA showed significant increase in NL% after 6M (p<0.05). Dentin bonds made with 2% PA/10% phosphoric acid showed no increase in NL% after 6 months. The MMP activity within the resin-dentin interface was almost completely reduced after 2% PA/10% PhA etching, while the 35% PhA exhibited intense MMP activity. For μSBS, the type of etchant and the storage period did not affect the resin-enamel bond strengths (p>0.05). SIGNIFICANCE: Ten percent phosphoric acid containing 2% PA can produce stable resin-dentin and enamel-resin interfaces, without requiring additional steps in the bonding procedure. Future studies for longer evaluation time are required.
OBJECTIVES: This study evaluated the effect of etching using 2% proanthocynidin-containing 10% phosphoric acid 2% PA/10% PhA vs. 35% phosphoric acid 35% PhA on immediate (IM) and 6-months (6M) resin-enamel microshear bond strength (μSBS), resin-dentin microtensile bond strength (μTBS), nanoleakage (NL) and as well as in situ MMP inhibition potential. METHODS: The dentin surface of human were exposed and then etched using 35% phosphoric acid for 15s or 2% PA/10% phosphoric acid for 30s. After rinsing with water, the dentin was bonded with Single Bond Plus (3M ESPE) and composite build-ups were constructed, followed by polymerization. The teeth were sectioned and the bonds were testing for microtensile bond strength (μTBS) and by SEM for NL analysis at IM and 6M. For MMP activity, resin-dentin slices were prepared for in situ zymography, and analyzed under confocal microscopy. For μSBS, others teeth had flattened enamel surfaces etched according the experimental groups and prepared to microshear procedure. The specimens were tested IM and after 6M by microshear bond strength. The data were submitted to two-way repeated measures ANOVA and Tukey's test (α=0.05). RESULTS: Acid-etching using the 2% PA/10% phosphoric acid did not lower the μTBS in IM (p>0.05) compared to the control 35% phosphoric acid group. However, after 6M, only the 2% PA/10% PhA etched dentin had remained stable the resin-dentin bond strength (p<0.05). Bonds made with 35% PhA showed significant increase in NL% after 6M (p<0.05). Dentin bonds made with 2% PA/10% phosphoric acid showed no increase in NL% after 6 months. The MMP activity within the resin-dentin interface was almost completely reduced after 2% PA/10% PhA etching, while the 35% PhA exhibited intense MMP activity. For μSBS, the type of etchant and the storage period did not affect the resin-enamel bond strengths (p>0.05). SIGNIFICANCE: Ten percent phosphoric acid containing 2% PA can produce stable resin-dentin and enamel-resin interfaces, without requiring additional steps in the bonding procedure. Future studies for longer evaluation time are required.
Authors: Mackeler Ramos Polassi; Thales de Sá Oliveira; Ana Calheiros de Carvalho; Lívia Soman de Medeiros Medeiros; Thiago André Moura Veiga; Carlos Frederico de Oliveira Graeff; Alejandra Hortencia Miranda González; Maria Cristina Marcucci; Simone Dos Santos Grecco; Paulo Henrique Perlatti D'Alpino Journal: ScientificWorldJournal Date: 2021-06-15