Jianru Yi1, Boxi Yan2, Meile Li1, Yu Wang3, Wei Zheng4, Yu Li5, Zhihe Zhao1. 1. Department of Orthodontics, State Key Laboratory of Oral Diseases, West China School and Hospital of Stomatology, Sichuan University, Chengdu, China. 2. Department of Orthodontics, School and Hospital of Stomatology, Peking University, Beijing, China. 3. Department of Orthodontics, Guanghua School and Hospital of Stomatology, Sun yat-sen University, Guangzhou, China. 4. Department of Oral and Maxillofacial Surgery, State Key Laboratory of Oral Diseases, West China School and Hospital of Stomatology, Sichuan University, Chengdu, China. 5. Department of Orthodontics, State Key Laboratory of Oral Diseases, West China School and Hospital of Stomatology, Sichuan University, Chengdu, China. Electronic address: yuli@scu.edu.cn.
Abstract
OBJECTIVE: Caffeine is the kernel component of coffee and has multiple effects on bone metabolism. Here we aimed to investigate the effects of caffeine intake on orthodontic tooth movement (OTM). DESIGN: (1) In the in vivo study, two groups comprising 15 randomly assigned rats each underwent orthodontic treatment. One group ingested caffeine at 25mg/kg body weight per day and the other, plain water. After 3 weeks, the degree of tooth movement and effect on the periodontium were assessed. (2) In the in vitro study, we established a model mimicking the essential bioprocess of OTM, which contained a periodontal ligament tissue model (PDLtm), and a co-culture system of osteoblasts (OBs) and osteoclast precursors (pre-OCs). After being subjected to static compressive force with or without caffeine administration, the conditioned media from the PDLtm were used for the OB/pre-OC co-cultures to induce osteoclastogenesis. RESULTS: (1) In vivo, the caffeine group displayed a significantly greater rate of tooth movement than the control. The alveolar bone mineral density and bone volume fraction were similar between the two groups; however, immunohistochemical staining showed that the caffeine group had significantly more TRAP(+) osteoclasts and higher RANKL expression in the compressed periodontium. (2) In vitro, caffeine at 0.01mM significantly enhanced the compression-induced expression of RANKL and COX-2, as well as prostaglandin E2 production in the PDLtm. Furthermore, the "caffeine+compression"-conditioned media induced significantly more TRAP(+) OC formation when compared with compression alone. CONCLUSIONS: Daily intake of caffeine, at least at some specific dosage, may enhance OTM through increasing osteoclastogenesis.
OBJECTIVE:Caffeine is the kernel component of coffee and has multiple effects on bone metabolism. Here we aimed to investigate the effects of caffeine intake on orthodontic tooth movement (OTM). DESIGN: (1) In the in vivo study, two groups comprising 15 randomly assigned rats each underwent orthodontic treatment. One group ingested caffeine at 25mg/kg body weight per day and the other, plain water. After 3 weeks, the degree of tooth movement and effect on the periodontium were assessed. (2) In the in vitro study, we established a model mimicking the essential bioprocess of OTM, which contained a periodontal ligament tissue model (PDLtm), and a co-culture system of osteoblasts (OBs) and osteoclast precursors (pre-OCs). After being subjected to static compressive force with or without caffeine administration, the conditioned media from the PDLtm were used for the OB/pre-OC co-cultures to induce osteoclastogenesis. RESULTS: (1) In vivo, the caffeine group displayed a significantly greater rate of tooth movement than the control. The alveolar bone mineral density and bone volume fraction were similar between the two groups; however, immunohistochemical staining showed that the caffeine group had significantly more TRAP(+) osteoclasts and higher RANKL expression in the compressed periodontium. (2) In vitro, caffeine at 0.01mM significantly enhanced the compression-induced expression of RANKL and COX-2, as well as prostaglandin E2 production in the PDLtm. Furthermore, the "caffeine+compression"-conditioned media induced significantly more TRAP(+) OC formation when compared with compression alone. CONCLUSIONS: Daily intake of caffeine, at least at some specific dosage, may enhance OTM through increasing osteoclastogenesis.
Authors: Lukas Entenmann; Maik Pietzner; Anna Artati; Anke Hannemann; Ann-Kristin Henning; Gabi Kastenmüller; Henry Völzke; Matthias Nauck; Jerzy Adamski; Henri Wallaschofski; Nele Friedrich Journal: PLoS One Date: 2017-09-18 Impact factor: 3.240
Authors: Marwa M S Abbass; Dina Rady; Israa Ahmed Radwan; Sara El Moshy; Nermeen AbuBakr; Mohamed Ramadan; Nermin Yussif; Ayoub Al Jawaldeh Journal: F1000Res Date: 2019-10-11