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Literature DB >> 26771404

Development of a Neutralizing Monoclonal Antibody Against Porcine Epidemic Diarrhea Virus S1 Protein.

Yunjing Zhang¹, Yali Yao¹, Xiaojing Gao¹, Yanhui Wang¹, Xiangrui Jia¹, Yan Xiao¹, Tongyan Wang¹, Xiangdong Li¹, Kegong Tian^1,2.

Abstract

The truncated spike protein (S1, 390-789aa) of porcine epidemic diarrhea virus was expressed as a recombinant protein in Baculorvirus expression system. Female BALB/c mice were immunized with the purified recombinant S1 (rS1) protein and two monoclonal antibodies, designated 8A3A10 and 12C4G12, were generated by hybridoma technique. The two monoclonal antibodies specifically reacted with PEDV rS1 protein, as proven by Western blot and indirect immunofluorescence assay. 8A3A10 monoclonal antibody showed a neutralizing activity to PEDV, as demonstrated in the results of fluorescent focus neutralization assay. These two newly established monoclonal antibodies may work as a useful tool to study the pathogenic mechanisms of PED virus and to develop diagnostic reagents.

Entities: Disease Gene Species

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Year: 2016 PMID： 26771404 DOI： 10.1089/mab.2015.0049

Source DB: PubMed Journal: Monoclon Antib Immunodiagn Immunother ISSN： 2167-9436

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Cited

6 in total

1. Cell Attachment Domains of the Porcine Epidemic Diarrhea Virus Spike Protein Are Key Targets of Neutralizing Antibodies.

Authors: Chunhua Li; Wentao Li; Eduardo Lucio de Esesarte; Hongbo Guo; Paul van den Elzen; Eduard Aarts; Erwin van den Born; Peter J M Rottier; Berend-Jan Bosch
Journal: J Virol Date: 2017-05-26 Impact factor: 5.103

2. The S Gene Is Necessary but Not Sufficient for the Virulence of Porcine Epidemic Diarrhea Virus Novel Variant Strain BJ2011C.

Authors: Di Wang; Xinna Ge; Dongjie Chen; Jie Li; Yueqi Cai; Jin Deng; Lei Zhou; Xin Guo; Jun Han; Hanchun Yang
Journal: J Virol Date: 2018-06-13 Impact factor: 5.103

3. Rapid Transient Production of a Monoclonal Antibody Neutralizing the Porcine Epidemic Diarrhea Virus (PEDV) in Nicotiana benthamiana and Lactuca sativa.

Authors: Kaewta Rattanapisit; Anchalee Srijangwad; Taksina Chuanasa; Suchada Sukrong; Angkana Tantituvanont; Hugh S Mason; Dachrit Nilubol; Waranyoo Phoolcharoen
Journal: Planta Med Date: 2017-06-02 Impact factor: 3.352

Development of a Neutralizing Monoclonal Antibody Against Porcine Epidemic Diarrhea Virus S1 Protein.

1. Cell Attachment Domains of the Porcine Epidemic Diarrhea Virus Spike Protein Are Key Targets of Neutralizing Antibodies.

2. The S Gene Is Necessary but Not Sufficient for the Virulence of Porcine Epidemic Diarrhea Virus Novel Variant Strain BJ2011C.

3. Rapid Transient Production of a Monoclonal Antibody Neutralizing the Porcine Epidemic Diarrhea Virus (PEDV) in Nicotiana benthamiana and Lactuca sativa.

4. A therapeutic chimeric IgG/IgA expressed by CHO cells for oral treatment of PED in piglets.

5. Neutralizing antibodies against porcine epidemic diarrhea virus block virus attachment and internalization.

Review 6. Cellular entry of the porcine epidemic diarrhea virus.