Guo-Ping Zhang1, Jing-Na Sun2, Jian Wang1, Hai-Jing Zhang1, Chao-Hua Zhu1, Lei Lin1, Quan-Hai Li3, Zhen-Shuan Zhao1, Xiao-Guang Yu1, Guo-Bin Liu1, Wei Dong1. 1. Department of Orthopeadic Surgery, The First Hospital of Hebei Medical University No. 89, Donggang Road, Yuhua District, Shijiazhuang, Hebei Province, China. 2. Department of Clinical Laboratory, The First Hospital of Hebei Medical University No. 89, Donggang Road, Yuhua District, Shijiazhuang, Hebei Province, China. 3. Department of Cell Therapy Center, The First Hospital of Hebei Medical University No. 89 Donggang Road, Yuhua District, Shijiazhuang, Hebei Province, China.
Abstract
OBJECTIVE: We analyzed the correlation between mutation in intron 4 and exon 7 of endothelial nitric oxide synthase (eNOS) and avascular necrosis of femoral head (ANFH). METHOD: A total of 260 ANFH cases without history of hip joint injuries were diagnosed and subject to staging according to Ficat standard, with 262 health subjects as control. Venous blood was collected to extract genome DNA, which was then amplified by PCR. The polymorphism of 27 bp repeat sequence in intron 4 and G894T polymorphism in exon 7 of eNOS gene was detected. RESULTS: The b/b, b/a and a/a genotype frequency of intron 4 was 77.7%, 19.2% and 3.1% in ANFH group, respectively, and that in the control group was 58.0%, 32.8% and 9.2%, respectively. The b allele frequency in ANFH group was obviously higher than that in the control (P<0.0001). The frequency of 894 G/G wild type, G/T heterozygote and T/T homozygote in eNOS exon 7 was analyzed by PCR-RLFP: 65.4%, 26.5% and 8.1% in ANFH group, and 46.2%, 37.8% and 16% in normal control, respectively. The frequency of TT genotype in ANFH was obviously higher than that in the control group (P<0.001). CONCLUSION: Polymorphism of eNOS was correlated with ANFH.
OBJECTIVE: We analyzed the correlation between mutation in intron 4 and exon 7 of endothelial nitric oxide synthase (eNOS) and avascular necrosis of femoral head (ANFH). METHOD: A total of 260 ANFH cases without history of hip joint injuries were diagnosed and subject to staging according to Ficat standard, with 262 health subjects as control. Venous blood was collected to extract genome DNA, which was then amplified by PCR. The polymorphism of 27 bp repeat sequence in intron 4 and G894T polymorphism in exon 7 of eNOS gene was detected. RESULTS: The b/b, b/a and a/a genotype frequency of intron 4 was 77.7%, 19.2% and 3.1% in ANFH group, respectively, and that in the control group was 58.0%, 32.8% and 9.2%, respectively. The b allele frequency in ANFH group was obviously higher than that in the control (P<0.0001). The frequency of 894 G/G wild type, G/T heterozygote and T/T homozygote in eNOS exon 7 was analyzed by PCR-RLFP: 65.4%, 26.5% and 8.1% in ANFH group, and 46.2%, 37.8% and 16% in normal control, respectively. The frequency of TT genotype in ANFH was obviously higher than that in the control group (P<0.001). CONCLUSION: Polymorphism of eNOS was correlated with ANFH.
Authors: Kou-Ti Peng; Kuo-Chin Huang; Tsan-Wen Huang; Yun-Shien Lee; Wei-Hsiu Hsu; Robert W W Hsu; Steve W N Ueng; Mel S Lee Journal: PLoS One Date: 2014-08-13 Impact factor: 3.240
Authors: Emma Paderno; Vincenzo Zanon; Giuliano Vezzani; Tommaso Antonio Giacon; Thomas L Bernasek; Enrico M Camporesi; Gerardo Bosco Journal: Int J Environ Res Public Health Date: 2021-03-12 Impact factor: 3.390