Xuan He1, Shuang Li2, Xiaoji Luo3, Dongyu Hu1, Tao Cai1, Kun Huang1, Weikang Zhou2, Jin Chen1. 1. Department of Dermatology, The First Affiliated Hospital of Chongqing Medical University Chongqing, China. 2. Department of Dermatology, Chongqing Third People's Hospital Chongqing, China. 3. Department of Orthopaedic, The First Affiliated Hospital of Chongqing Medical University Chongqing, China.
Abstract
OBJECTIVE: To investigate the expression of Dickkopf-1 (DKK1) and β-catenin and their correlation in epidermal neoplasms. METHODS: Immunohistochemical staining was applied to detect the expression of DKK1 and β-catenin in tissue samples of 19 cases of seborrheic keratosis (SK), 16 cases of actinic keratosis (AK), 24 cases of Bowen's disease (BD), 25 cases of cutaneous squamous cell carcinoma (SCC), and 22 cases of normal epidermal tissue (NET). RESULTS: DKK1 was expressed in cytoplasm in normal epidermis. The positive expression rates of DKK1 in SK, AK, BD and SCC were 63.16%, 50.00%, 12.50% and 8.00%, respectively. β-catenin was expressed in cell membrane in normal epidermis. The abnormal expression rates of β-catenin in SK, AK, BD and SCC were 15.79%, 56.25%, 91.67% and 96.00%, respectively. Additionally, significant negative correlation was observable between the expression of β-catenin and DKK1 (r=-0.692, P=0.000). CONCLUSION: The Wnt signaling pathway may play an important role in the process of epidermal neoplasms formation. The loss of DKK1 promotes the abnormal expression of β-catenin through Wnt signaling pathway.
OBJECTIVE: To investigate the expression of Dickkopf-1 (DKK1) and β-catenin and their correlation in epidermal neoplasms. METHODS: Immunohistochemical staining was applied to detect the expression of DKK1 and β-catenin in tissue samples of 19 cases of seborrheic keratosis (SK), 16 cases of actinic keratosis (AK), 24 cases of Bowen's disease (BD), 25 cases of cutaneous squamous cell carcinoma (SCC), and 22 cases of normal epidermal tissue (NET). RESULTS:DKK1 was expressed in cytoplasm in normal epidermis. The positive expression rates of DKK1 in SK, AK, BD and SCC were 63.16%, 50.00%, 12.50% and 8.00%, respectively. β-catenin was expressed in cell membrane in normal epidermis. The abnormal expression rates of β-catenin in SK, AK, BD and SCC were 15.79%, 56.25%, 91.67% and 96.00%, respectively. Additionally, significant negative correlation was observable between the expression of β-catenin and DKK1 (r=-0.692, P=0.000). CONCLUSION: The Wnt signaling pathway may play an important role in the process of epidermal neoplasms formation. The loss of DKK1 promotes the abnormal expression of β-catenin through Wnt signaling pathway.
Authors: H Suzuki; M Toyota; H Carraway; H Caraway; E Gabrielson; T Ohmura; T Fujikane; N Nishikawa; Y Sogabe; M Nojima; T Sonoda; M Mori; K Hirata; K Imai; Y Shinomura; S B Baylin; T Tokino Journal: Br J Cancer Date: 2008-02-19 Impact factor: 7.640