Fen Ai1, Manhua Chen2, Wei Li1, Yang Yang1, Guizhong Xu1, Feng Gui1, Zhenxing Liu1, Xiangyan Bai1, Zhen Chen1. 1. Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science & Technology Wuhan 430014, Hubei Province, China. 2. Department of Cardiology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science & Technology Wuhan 430014, Hubei Province, China.
Abstract
OBJECTIVE: The traditional Chinese medicinal Danshen (Salvia miltiorrhiza), has long been used to treat cardiovascular diseases, however, the mechanism underlying its effects remain unclear. Here, this study would to investigate the effects of Danshen injection on myocardial infarction-induced cardiac damage. METHODS: Danshen was injected into mice models at low dose (3 g/kg per day) or at high dose (6 g/kg per day) after left anterior descending coronary artery (LAD) ligation. After 4 weeks, cardiac function was evaluated by echocardiography. Paraffin sections of the hearts were used for isolectin GS-IB4 staining. Protein and mRNA expression levels of HIF1α and VEGFA were evaluated by western blotting and real-time polymerase chain reaction (RT-PCR). RESULTS: The hearts showed significantly impaired angiogenesis and slightly increase of HIF1α and VEGFA expression after LAD ligation. The angiogenesis defect and heart failure were partially rescued in Danshen treatment mice with great increase of HIF1α and VEGFA mRNA levels and protein expression. CONCLUSION: These results illustrated that the protective effects of Danshen injection in responsive cardiac angiogenesis were at least in part due to increased HIF1α and VEGFA expression.
OBJECTIVE: The traditional Chinese medicinal Danshen (Salvia miltiorrhiza), has long been used to treat cardiovascular diseases, however, the mechanism underlying its effects remain unclear. Here, this study would to investigate the effects of Danshen injection on myocardial infarction-induced cardiac damage. METHODS: Danshen was injected into mice models at low dose (3 g/kg per day) or at high dose (6 g/kg per day) after left anterior descending coronary artery (LAD) ligation. After 4 weeks, cardiac function was evaluated by echocardiography. Paraffin sections of the hearts were used for isolectin GS-IB4 staining. Protein and mRNA expression levels of HIF1α and VEGFA were evaluated by western blotting and real-time polymerase chain reaction (RT-PCR). RESULTS: The hearts showed significantly impaired angiogenesis and slightly increase of HIF1α and VEGFA expression after LAD ligation. The angiogenesis defect and heart failure were partially rescued in Danshen treatment mice with great increase of HIF1α and VEGFA mRNA levels and protein expression. CONCLUSION: These results illustrated that the protective effects of Danshen injection in responsive cardiac angiogenesis were at least in part due to increased HIF1α and VEGFA expression.
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