Yan-Yun Wang1, Rong Zhou2, Bin Zhou1, Tao Wang1, Lin Zhang1, Dong Luo3. 1. Laboratory of Molecular Translational Medicine, West China Institute of Women and Children's Health, Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University Chengdu 610041, Sichuan, China. 2. Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University Chengdu 610041, Sichuan, China. 3. Department of Anesthesiology, West China Second University Hospital, Sichuan University Chengdu 610041, Sichuan, China.
Abstract
BACKGROUND: Preeclampsia is associated with inadequate invasion of trophocytes and spiral artery remodeling. As a β-D-glucuronidase enzyme, Heparanase is related to tumor angiogenesis, development and invasion. Trophocytes have similar characteristics to tumor cells, and heparanase could therefore play an important role in the pathogenesis of preeclampsia. METHODS: The expression of heparanase in severe preeclampsia and normal placentas was detected via real-time PCR, immunohistochemistry and western blotting. The effects of heparanase on trophocytes migration and invasion were investigated by culturing the HTR-8/Svneo cell line with recombinant human heparanase protein in vitro. RESULTS: The levels of inactive 65-kDa heterologous heparanase dimers were obviously increased, and the content of the 50-kDa active polypeptide was decreased in severe preeclampsia. Furthermore, exogenous heparanase protein could reduce the migration and invasion of HTR-8/Svneo cells. CONCLUSION: Our results suggested that heparanase might be an important factor in the pathogenesis of severe preeclampsia.
BACKGROUND: Preeclampsia is associated with inadequate invasion of trophocytes and spiral artery remodeling. As a β-D-glucuronidase enzyme, Heparanase is related to tumor angiogenesis, development and invasion. Trophocytes have similar characteristics to tumor cells, and heparanase could therefore play an important role in the pathogenesis of preeclampsia. METHODS: The expression of heparanase in severe preeclampsia and normal placentas was detected via real-time PCR, immunohistochemistry and western blotting. The effects of heparanase on trophocytes migration and invasion were investigated by culturing the HTR-8/Svneo cell line with recombinant humanheparanase protein in vitro. RESULTS: The levels of inactive 65-kDa heterologous heparanase dimers were obviously increased, and the content of the 50-kDa active polypeptide was decreased in severe preeclampsia. Furthermore, exogenous heparanase protein could reduce the migration and invasion of HTR-8/Svneo cells. CONCLUSION: Our results suggested that heparanase might be an important factor in the pathogenesis of severe preeclampsia.
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