Qian-Li Dang1, Jian-Gang Li2, Yang Sun1, Zhan-Kui Jin3, Ying Gao4, Cui-Xiang Xu5, Ping Chen1, Ting Ma1, Jiang-Cun Yang1. 1. Department of Transfusion Medicine, The Third Affiliated Hospital of Medical College of Xi'an Jiaotong University Xi'an 710068, China. 2. Department of Transfusion Medicine, Xi'an No. 4 Hospital Xi'an 710004, China. 3. The Second Department of Orthopaedics, The Third Affiliated Hospital of Medical College of Xi'an Jiaotong University Xi'an 710068, China. 4. Department of Hematology, The Third Affiliated Hospital of Medical College of Xi'an Jiaotong University Xi'an 710068, China. 5. Shaanxi Provincial Center for Clinical Laboratory, The Third Affiliated Hospital of Medical College of Xi'an Jiaotong University Xi'an 710068, China.
Abstract
OBJECTIVE: To provide a basis for the cold-storage of human platelets as a way to assess changes in platelet function. METHODS: Red blood cell suspensions (11 U and 50 U) were randomly selected at different storage times (3-28 days) and evidence of platelet activation (CD62P) and thromboelastography (TEG) reaction times were investigated. RESULTS: After 21 days of storage at 4°C, a large number of activated platelets (PAC1+62P+, PAC1-62P+) within the red blood cell suspension (RBCs) retained their function and had TEG-maximum amplitude (TEG-MA) indices in the normal range. CONCLUSION: We report that platelets in RBC suspensions retain high activity when stored at 4°C for 21 days. The results provide important information for studies that involve storing platelets under cold conditions.
OBJECTIVE: To provide a basis for the cold-storage of human platelets as a way to assess changes in platelet function. METHODS: Red blood cell suspensions (11 U and 50 U) were randomly selected at different storage times (3-28 days) and evidence of platelet activation (CD62P) and thromboelastography (TEG) reaction times were investigated. RESULTS: After 21 days of storage at 4°C, a large number of activated platelets (PAC1+62P+, PAC1-62P+) within the red blood cell suspension (RBCs) retained their function and had TEG-maximum amplitude (TEG-MA) indices in the normal range. CONCLUSION: We report that platelets in RBC suspensions retain high activity when stored at 4°C for 21 days. The results provide important information for studies that involve storing platelets under cold conditions.
Authors: P F van der Meer; J-L Kerkhoffs; J Curvers; J Scharenberg; D de Korte; A Brand; J de Wildt-Eggen Journal: Vox Sang Date: 2009-11-18 Impact factor: 2.144
Authors: C R Valeri; G Cassidy; L E Pivacek; G Ragno; W Lieberthal; J P Crowley; S F Khuri; J Loscalzo Journal: Transfusion Date: 2001-08 Impact factor: 3.157