Draft Genome Sequence of Streptococcus orisasini SH06, Isolated from a Healthy Thoroughbred Gastrointestinal Tract.

Streptococcus orisasini SH06 was isolated from a healthy thoroughbred gastrointestinal tract. Here, we report the draft genome sequence of this organism. This paper is the first published report of the genomic sequence of S. orisasini.

GENOME ANNOUNCEMENT

Horses have a large and complex gastrointestinal tract. We have previously isolated lactobacilli from feces of nine thoroughbreds (1). During this work, we have also isolated coccoid forms of bacterium (strain SH06) from a healthy thoroughbred (male, 4-year-old). We performed 16S rRNA gene sequencing and thus confirmed that strain SH06 belongs to Streptococcus orisasini, which was reported as a novel species in 2013 (2). We deposited S. orisasini SH06 in the Japan Collection of Microorganisms (JCM) as strain JCM 17305.

The S. orisasini SH06 genome was paired-end sequenced using Illumina’s MiSeq platform. Genomic libraries containing 600 to 1,000 bp inserts were constructed and sequenced, yielding 3,509,312 sequences that provided 346-fold coverage from both ends of the genomic clones. The sequence reads were assembled using Newbler version 2.8 (Roche), and the assembled genome consists of 67 contigs with a total length of 2,299,160 bp. The draft genome of S. orisasini SH06 contained 2,099 predicted protein-coding genes, most of which shared high sequence homology to those of Streptococcus mutans, which is commonly found in the human oral cavity. The type strain of S. orisasini is isolated from the oral cavity of donkeys (2). Thus, S. orisasini might be a member of horse oral microbiota.

Several Streptococcus species, such as S. equi, S. bovis, S. zooepidemicus, S. dysgalactiae subsp. equisimilis, S. pneumoniae (capsule type III), S. henryi, and S. caballi, have been isolated from the horse gastrointestinal tract as pathogens (3–7). On the other hand, S. orisasini SH06 is likely to be a nonpathogenic strain, because this strain was isolated from a healthy thoroughbred gastrointestinal tract. Thus, the genome information of this species will be useful for further studies of the pathogenicity of Streptococcus for equine species.

Nucleotide sequence accession numbers.

The draft genome sequence for S. orisasini SH06 (= JCM 17305) has been deposited in the DDBJ/GenBank/EMBL database under the accession numbers BCLE01000001 to BCLE01000067.