Karim M Fawzy El-Sayed1, Pauline Klingebiel2, Christof E Dörfer2. 1. Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian Albrechts University, Kiel, Germany; Oral Medicine and Periodontology Department, Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt. Electronic address: karim.fawzy@gmail.com. 2. Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian Albrechts University, Kiel, Germany.
Abstract
INTRODUCTION: Human dental pulp stem/progenitor cells (DPSCs) show remarkable regenerative potential in vivo. During regeneration, DPSCs may interact with their inflammatory environment via toll-like receptors (TLRs). The present study aimed to depict for the first time the TLR expression profile of DPSCs. METHODS: Cells were isolated from human dental pulp, STRO-1-immunomagnetically sorted, and seeded out to obtain single colony-forming units. DPSCs were characterized for CD14, CD34, CD45, CD73, CD90, CD105, and CD146 expression and for their multilineage differentiation potential. After incubation of DPSCs in basic or inflammatory medium (interleukin-1β, interferon-γ, interferon-α, tumor necrosis factor-α), TLR expression profiles were generated (DPSCs and DPSCs-i). RESULTS: DPSCs showed all characteristics of stem/progenitor cells. In basic medium DPSCs expressed TLRs 1-10 in different quantities. The inflammatory medium upregulated the expression of TLRs 2, 3, 4, 5, and 8, downregulated TLRs 1, 7, 9, and 10, and abolished TLR6. CONCLUSIONS: The current study describes for the first time the distinctive TLR expression profile of DPSCs in uninflamed and inflamed conditions.
INTRODUCTION:Human dental pulp stem/progenitor cells (DPSCs) show remarkable regenerative potential in vivo. During regeneration, DPSCs may interact with their inflammatory environment via toll-like receptors (TLRs). The present study aimed to depict for the first time the TLR expression profile of DPSCs. METHODS: Cells were isolated from human dental pulp, STRO-1-immunomagnetically sorted, and seeded out to obtain single colony-forming units. DPSCs were characterized for CD14, CD34, CD45, CD73, CD90, CD105, and CD146 expression and for their multilineage differentiation potential. After incubation of DPSCs in basic or inflammatory medium (interleukin-1β, interferon-γ, interferon-α, tumor necrosis factor-α), TLR expression profiles were generated (DPSCs and DPSCs-i). RESULTS: DPSCs showed all characteristics of stem/progenitor cells. In basic medium DPSCs expressed TLRs 1-10 in different quantities. The inflammatory medium upregulated the expression of TLRs 2, 3, 4, 5, and 8, downregulated TLRs 1, 7, 9, and 10, and abolished TLR6. CONCLUSIONS: The current study describes for the first time the distinctive TLR expression profile of DPSCs in uninflamed and inflamed conditions.
Authors: Xenos Petridis; Luc W M van der Sluis; René J B Dijkstra; Marja G L Brinker; Henny C van der Mei; Martin C Harmsen Journal: Sci Rep Date: 2018-08-21 Impact factor: 4.379
Authors: Matthias Widbiller; Helmut Schweikl; Astrid Bruckmann; Andreas Rosendahl; Eduard Hochmuth; Sophia R Lindner; Wolfgang Buchalla; Kerstin M Galler Journal: Sci Rep Date: 2019-03-14 Impact factor: 4.379