Yinling Zhuo1, Qisen Guo2, Pingping Song3, Qiong Zhang4, Chen Guo5, Hongsheng Zeng6, Yan Guan2, Xiuju Liu2, Chenqing Zhao7. 1. Department of Internal medicine, Shandong Academy of Occupational Health and Occupational Medicine Jinan, China. 2. Department of Chemotherapy Oncology, Shandong Cancer Hospital Jinan, China. 3. Department of Thoracic Surgery, Shandong Cancer Hospital Jinan, China. 4. Department of Internal Medicine, Shandong Traffic Hospital Jinan, China. 5. Department of Oncology, Affiliated Hospital of Binzhou Medical College Binzhou, China. 6. Department of Oncology, Jinxiang People's Hospital Jinxiang, China. 7. Department of Internal Medicine, Affiliated Hospital of Shandong Academy of Medical Sciences Jinan, China.
Abstract
BACKGROUND: This study was designed to detect the protein expression of epidermal growth factor receptor (EGFR) among serum, lymph node, and tumor tissues, and to discuss their relationship and clinical significance. We investigated whether EGFR levels in serum and lymph nodes could be used as an effective method for non-small cell lung cancer (NSCLC) to diagnose and assess clinical stage. METHODS: In 56 patients with NSCLC and 10 individuals with nonmalignant thoracic disease, we measured EGFR levels in serum using an enzyme immunoassay, and EGFR mRNA levels in lymph node and NSCLC tissues by quantitative real-time-polymerase chain reaction. We examined the correlation between them and with the clinical parameters. RESULTS: Serum EGFR levels substantially decreased after surgical treatment (P < 0.001). Serum EGFR levels were correlated with smoking, surgery, and pathological type after surgery (all P < 0.05). EGFR mRNA levels in lymph node and tumor tissues were correlated more closely with lymph node metastasis (P = 0.015. EGFR mRNA in tissues was higher than that of benign pulmonary diseases (P = 0.020). There was an obvious positive correlation among EGFR levels of serum and lymph node tissues (r = 0.764; P < 0.001), serum and tumor tissues (r = 0.616; P < 0.001), and lymph node and tumor tissues (r = 0.904; P < 0.001) in NSCLCs. CONCLUSION: The data suggest that detecting EGFR levels in serum and lymph node tissues could be a simple and effective method to diagnose and assess the clinical stage in patients with NSCLC.
BACKGROUND: This study was designed to detect the protein expression of epidermal growth factor receptor (EGFR) among serum, lymph node, and tumor tissues, and to discuss their relationship and clinical significance. We investigated whether EGFR levels in serum and lymph nodes could be used as an effective method for non-small cell lung cancer (NSCLC) to diagnose and assess clinical stage. METHODS: In 56 patients with NSCLC and 10 individuals with nonmalignant thoracic disease, we measured EGFR levels in serum using an enzyme immunoassay, and EGFR mRNA levels in lymph node and NSCLC tissues by quantitative real-time-polymerase chain reaction. We examined the correlation between them and with the clinical parameters. RESULTS: Serum EGFR levels substantially decreased after surgical treatment (P < 0.001). Serum EGFR levels were correlated with smoking, surgery, and pathological type after surgery (all P < 0.05). EGFR mRNA levels in lymph node and tumor tissues were correlated more closely with lymph node metastasis (P = 0.015. EGFR mRNA in tissues was higher than that of benign pulmonary diseases (P = 0.020). There was an obvious positive correlation among EGFR levels of serum and lymph node tissues (r = 0.764; P < 0.001), serum and tumor tissues (r = 0.616; P < 0.001), and lymph node and tumor tissues (r = 0.904; P < 0.001) in NSCLCs. CONCLUSION: The data suggest that detecting EGFR levels in serum and lymph node tissues could be a simple and effective method to diagnose and assess the clinical stage in patients with NSCLC.
Authors: Binaifer R Balsara; Jianming Pei; Yasuhiro Mitsuuchi; Robert Page; Andres Klein-Szanto; Hao Wang; Michael Unger; Joseph R Testa Journal: Carcinogenesis Date: 2004-07-07 Impact factor: 4.944
Authors: Ivan Milas; Ritsuko Komaki; Tsutomu Hachiya; Robbin S Bubb; Jae Y Ro; Lauren Langford; Raymond Sawaya; Joe B Putnam; Pamela Allen; James D Cox; Timothy J McDonnell; William Brock; Waun Ki Hong; Jack A Roth; Luka Milas Journal: Clin Cancer Res Date: 2003-03 Impact factor: 12.531