Ugur Acar1, Damla Erginturk Acar2, Cafer Tanriverdi3, Mutlu Acar4, Ozdemir Ozdemir2, Acelya Erikci5, Firdevs Ornek6. 1. a Department of Ophthalmology , Hacettepe University, Faculty of Medicine , Ankara , Turkey. 2. b Department of Ophthalmology , Zekai Tahir Burak Women's Health Research and Education Hospital , Ankara , Turkey. 3. c Department of Ophthalmology , İstanbul Medipol University , İstanbul , Turkey. 4. d Department of Ophthalmology , Dışkapı Yıldırım Beyazıt Research and Education Hospital , Ankara , Turkey. 5. e Department of Biochemistry , Hacettepe University, Faculty of Pharmacy , Ankara , Turkey. 6. f Department of Ophthalmology , Ankara Research and Education Hospital , Ankara , Turkey.
Abstract
PURPOSE: To compare the lowering effects of ketorolac 0.4% and nepafenac 0.1% on aqueous and vitreous humor prostaglandin E2 (PGE2) levels in rabbits. METHODS: Ketorolac and nepafenac ophthalmic solutions were administered to the right eyes of 24 healthy rabbits after randomized division into two groups. The left eyes of these rabbits were considered as controls for the two groups. On the 4th day of the experiment, the samples were taken from the aqueous and vitreous humors of the rabbits bilaterally, and PGE2 levels were measured by an enzyme immune assay kit. RESULTS: Ketorolac and nepafenac achieved a statistically significant decrease (p<0.001, for each) in PGE2 levels in the aqueous (11.75 ± 6.15 and 14.75 ± 7.60 pg/mL, respectively) and the vitreous humor (6.58 ± 4.62 and 9.83 ± 4.55 pg/mL, respectively). CONCLUSIONS: Both ketorolac and nepafenac inhibited PGE2 levels in both the aqueous and vitreous humors of rabbits. Although PGE2-lowering effects were similar in the aqueous humor, nepafenac seemed to be more potent than ketorolac in the vitreous humor.
PURPOSE: To compare the lowering effects of ketorolac 0.4% and nepafenac 0.1% on aqueous and vitreous humor prostaglandin E2 (PGE2) levels in rabbits. METHODS:Ketorolac and nepafenac ophthalmic solutions were administered to the right eyes of 24 healthy rabbits after randomized division into two groups. The left eyes of these rabbits were considered as controls for the two groups. On the 4th day of the experiment, the samples were taken from the aqueous and vitreous humors of the rabbits bilaterally, and PGE2 levels were measured by an enzyme immune assay kit. RESULTS:Ketorolac and nepafenac achieved a statistically significant decrease (p<0.001, for each) in PGE2 levels in the aqueous (11.75 ± 6.15 and 14.75 ± 7.60 pg/mL, respectively) and the vitreous humor (6.58 ± 4.62 and 9.83 ± 4.55 pg/mL, respectively). CONCLUSIONS: Both ketorolac and nepafenac inhibited PGE2 levels in both the aqueous and vitreous humors of rabbits. Although PGE2-lowering effects were similar in the aqueous humor, nepafenac seemed to be more potent than ketorolac in the vitreous humor.
Authors: Dmitry V Chistyakov; Nadezhda V Azbukina; Alina A Astakhova; Sergei V Goriainov; Viktor V Chistyakov; Veronika V Tiulina; Viktoriia E Baksheeva; Vladislav I Kotelin; Elena V Fedoseeva; Andrey A Zamyatnin; Pavel P Philippov; Olga A Kiseleva; Alexander M Bessmertny; Ivan I Senin; Elena N Iomdina; Marina G Sergeeva; Evgeni Yu Zernii Journal: Metabolomics Date: 2020-02-12 Impact factor: 4.290
Authors: Dmitry V Chistyakov; Viktoriia E Baksheeva; Veronika V Tiulina; Sergei V Goriainov; Nadezhda V Azbukina; Olga S Gancharova; Eugene A Arifulin; Sergey V Komarov; Viktor V Chistyakov; Natalia K Tikhomirova; Andrey A Zamyatnin; Pavel P Philippov; Ivan I Senin; Marina G Sergeeva; Evgeni Yu Zernii Journal: Int J Mol Sci Date: 2020-01-21 Impact factor: 5.923