Anja Biltoft-Jensen1, Camilla T Damsgaard2, Elisabeth W Andersen3, Karin H Ygil4, Rikke Andersen4, Majken Ege4, Tue Christensen4, Anne-Vibeke Thorsen4, Inge Tetens4, Huaxing Wu5, Rikard Landberg6. 1. Division of Nutrition, National Food Institute, Technical University of Denmark, Søborg, Denmark; apbj@food.dtu.dk. 2. Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, Copenhagen, Denmark; 3. Department of Applied Mathematics and Computer Science, Technical University of Denmark, Lyngby, Denmark; 4. Division of Nutrition, National Food Institute, Technical University of Denmark, Søborg, Denmark; 5. Department of Food Science, Swedish University of Agricultural Sciences, Uppsala, Sweden; and. 6. Department of Food Science, Swedish University of Agricultural Sciences, Uppsala, Sweden; and Nutritional Epidemiology Unit, Institute of Environmental Medicine, Karolinska Institutet, Solna, Sweden.
Abstract
BACKGROUND:Whole-grain (WG) intake is important for human health, but accurate intake estimation is challenging. Use of a biomarker for WG intake provides a possible way to validate dietary assessment methods. OBJECTIVE: Our aim was to validate WG intake from 2 diets reported by children, using plasma alkylresorcinol (AR) concentrations, and to investigate the 3-mo reproducibility of AR concentrations and reported WG intake. METHODS:AR concentrations were analyzed in fasting blood plasma samples, and WG intake was estimated in a 7-d web-based diary by 750 participants aged 8-11 y in a 2 school meal × 3 mo crossover trial. Reported WG intake and plasma AR concentrations were compared when children ate their usual bread-based lunch (UBL) and when served a hot lunch meal (HLM). Correlations and cross-classification were used to rank subjects according to intake. The intraclass correlation coefficients (ICCs) between subjects' measurements at baseline and after the UBL were used to assess reproducibility. RESULTS: Correlations between reported WG wheat + rye intake and plasma AR were 0.40 and 0.37 (P < 0.001) for the UBL and the HLM diets, and 78% and 77% were classified in the same or adjacent quartiles for the UBL and HLM diets, respectively. The ICC over 3 mo was 0.47 (95% CI: 0.38, 0.55) for plasma total ARs and 0.64 (95% CI: 0.58, 0.70) for reported WG intake. Correlations were higher when using the AR C17:0 homolog as a biomarker, reflecting rye intake instead of plasma total ARs [UBL: r = 0.47; HLM: r = 0.43, P < 0.001; ICC = 0.51 (95% CI: 0.43, 0.59)]. CONCLUSIONS:Self-reported WG wheat + rye intake among children showed moderate correlations with plasma AR concentrations. Substantial intraindividual variation was found in WG intake and plasma AR concentrations. The AR homolog C17:0 may be used as a biomarker for WG intake when the WG intake primarily comes from rye as in the present study. This trial was registered at clinicaltrials.gov as NCT01457794.
RCT Entities:
BACKGROUND: Whole-grain (WG) intake is important for human health, but accurate intake estimation is challenging. Use of a biomarker for WG intake provides a possible way to validate dietary assessment methods. OBJECTIVE: Our aim was to validate WG intake from 2 diets reported by children, using plasma alkylresorcinol (AR) concentrations, and to investigate the 3-mo reproducibility of AR concentrations and reported WG intake. METHODS:AR concentrations were analyzed in fasting blood plasma samples, and WG intake was estimated in a 7-d web-based diary by 750 participants aged 8-11 y in a 2 school meal × 3 mo crossover trial. Reported WG intake and plasma AR concentrations were compared when children ate their usual bread-based lunch (UBL) and when served a hot lunch meal (HLM). Correlations and cross-classification were used to rank subjects according to intake. The intraclass correlation coefficients (ICCs) between subjects' measurements at baseline and after the UBL were used to assess reproducibility. RESULTS: Correlations between reported WG wheat + rye intake and plasma AR were 0.40 and 0.37 (P < 0.001) for the UBL and the HLM diets, and 78% and 77% were classified in the same or adjacent quartiles for the UBL and HLM diets, respectively. The ICC over 3 mo was 0.47 (95% CI: 0.38, 0.55) for plasma total ARs and 0.64 (95% CI: 0.58, 0.70) for reported WG intake. Correlations were higher when using the AR C17:0 homolog as a biomarker, reflecting rye intake instead of plasma total ARs [UBL: r = 0.47; HLM: r = 0.43, P < 0.001; ICC = 0.51 (95% CI: 0.43, 0.59)]. CONCLUSIONS: Self-reported WG wheat + rye intake among children showed moderate correlations with plasma AR concentrations. Substantial intraindividual variation was found in WG intake and plasma AR concentrations. The AR homolog C17:0 may be used as a biomarker for WG intake when the WG intake primarily comes from rye as in the present study. This trial was registered at clinicaltrials.gov as NCT01457794.
Authors: Marie T B Madsen; Anja P Biltoft-Jensen; Ellen Trolle; Lotte Lauritzen; Kim F Michaelsen; Camilla T Damsgaard Journal: Eur J Nutr Date: 2022-05-27 Impact factor: 4.865
Authors: B Amoutzopoulos; T Steer; C Roberts; J E Cade; C J Boushey; C E Collins; E Trolle; E J de Boer; N Ziauddeen; C van Rossum; E Buurma; D Coyle; P Page Journal: J Nutr Sci Date: 2018-04-02
Authors: Anna Karin Lindroos; Jessica Petrelius Sipinen; Cecilia Axelsson; Gisela Nyberg; Rikard Landberg; Per Leanderson; Marianne Arnemo; Eva Warensjö Lemming Journal: J Med Internet Res Date: 2019-10-04 Impact factor: 5.428
Authors: Amanda Cramer-Nielsen; Sidse Marie Sidenius Bestle; Anja Pia Biltoft-Jensen; Jeppe Matthiessen; Anne Dahl Lassen; Bodil Just Christensen; Sarah Jegsmark Gibbons; Ellen Trolle Journal: Nutrients Date: 2022-03-11 Impact factor: 5.717