OBJECTIVE: To compare the results for homocysteine concentration using chemiluminescence and HPLC methods in samples from school-age children. In addition, to determine the reference values for patients of this age group and assess the real prognostic value of homocysteine in healthy children. METHODS: A prospective observational study was undertaken to determine plasma levels of homocysteine using two different assays, HPLC and chemiluminescence, in 185 samples from school-age children living in Santo Andre, with no chronic or inflammatory diseases, and absence of pubertal development. RESULTS: The results were presented in percentiles and reference values were determined within this age group (7-9 years old). Homocysteine concentration ranged from 2.0 to 9.9 µmol/l (r = 0.821 and p < 0.001). CONCLUSIONS: It was verified that chemiluminescence is comparable to HPLC when both techniques are used to detect homocysteine in school-age children. There is an important correlation between both methods, which allows investigation of this amino acid as a risk factor for heart diseases.
OBJECTIVE: To compare the results for homocysteine concentration using chemiluminescence and HPLC methods in samples from school-age children. In addition, to determine the reference values for patients of this age group and assess the real prognostic value of homocysteine in healthy children. METHODS: A prospective observational study was undertaken to determine plasma levels of homocysteine using two different assays, HPLC and chemiluminescence, in 185 samples from school-age children living in Santo Andre, with no chronic or inflammatory diseases, and absence of pubertal development. RESULTS: The results were presented in percentiles and reference values were determined within this age group (7-9 years old). Homocysteine concentration ranged from 2.0 to 9.9 µmol/l (r = 0.821 and p < 0.001). CONCLUSIONS: It was verified that chemiluminescence is comparable to HPLC when both techniques are used to detect homocysteine in school-age children. There is an important correlation between both methods, which allows investigation of this amino acid as a risk factor for heart diseases.
Authors: Eliana K Yamashita; Bianca M Teixeira; Renata N Yoshihara; Renata K Kuniyoshi; Beatriz C A Alves; Flávia S Gehrke; Viviane A Vilas-Bôas; João A Correia; Ligia A Azzalis; Virginia B C Junqueira; Edimar Cristiano Pereira; Fernando L A Fonseca Journal: J Clin Lab Anal Date: 2014-01-06 Impact factor: 2.352
Authors: Renata Nunes Yoshihara; Bianca Marinelli Teixeira; Fernando Adami; Renata K Kuniyoshi; Beatriz C A Alves; Flávia S Gehrke; Viviane A Vilas-Bôas; Ligia A Azzalis; Virginia B C Junqueira; Edimar Cristiano Pereira; Fernando L A Fonseca Journal: Tumour Biol Date: 2013-05-19
Authors: Marcelo R Bacci; Ethel Z Chehter; Ligia A Azzalis; Beatriz Costa de Aguiar Alves; Fernando L A Fonseca Journal: Kidney Int Rep Date: 2016-10-15