Keewan Kim1, Michael S Bloom2,3, Victor Y Fujimoto4, Erin M Bell1, Recai M Yucel5, Richard W Browne6. 1. Department of Environmental Health Sciences, University at Albany, State University of New York, School of Public Health Rm. #157, One University Place, Rensselaer, 12144, NY, USA. 2. Department of Environmental Health Sciences, University at Albany, State University of New York, School of Public Health Rm. #157, One University Place, Rensselaer, 12144, NY, USA. mbloom@albany.edu. 3. Department of Epidemiology and Biostatistics, University at Albany, State University of New York, Rensselaer, NY, USA. mbloom@albany.edu. 4. Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California at San Francisco, San Francisco, CA, USA. 5. Department of Epidemiology and Biostatistics, University at Albany, State University of New York, Rensselaer, NY, USA. 6. Department of Biotechnical and Clinical Laboratory Sciences, University at Buffalo, State University of New York, Buffalo, NY, USA.
Abstract
PURPOSE: The purpose of this study was to examine the biological variability of follicular fluid (FF) high density lipoprotein (HDL) particle components measured in ipsilateral ovarian follicles. METHODS: We collected FF from two ipsilateral follicles among six women undergoing in vitro fertilization (IVF). We measured concentrations of 19 FF HDL particle components, including HDL cholesterol, free cholesterol, four cholesteryl esters, phospholipids, triglycerides, paraoxonase and arylesterase activities, apolipoproteins A-1 and A-2 (ApoA-1 and ApoA-2), and seven lipophilic micronutrients, by automated analysis and with high-performance liquid chromatography. We assessed biological variability using two-stage nested analysis of variance and compared values with those previously published for contralateral follicles. RESULTS: For most FF HDL analytes, there was little variability between follicles relative to the variability between women (i.e., %σ(2) F:%σ(2) B <0.5). Intraclass correlation coefficients were >0.80 for HDL cholesterol (0.82), phospholipids (0.89), paraoxonase (0.96), and arylesterase (0.91) activities, ApoA-1 (0.89), and ApoA-2 (0.90), and single specimen collections were required to estimate the subject-specific mean, demonstrating sufficient reliability for use as biomarkers of the follicular microenvironment in epidemiologic and clinical studies. CONCLUSIONS: These preliminary results raise the possibility for tighter regulation of HDL in follicles within the same ovary vs. between ovaries. Thus, collection of a single FF specimen may be sufficient to estimate HDL particle components concentrations within a single ovary. However, our results should be interpreted with caution as the analysis was based on a small sample.
PURPOSE: The purpose of this study was to examine the biological variability of follicular fluid (FF) high density lipoprotein (HDL) particle components measured in ipsilateral ovarian follicles. METHODS: We collected FF from two ipsilateral follicles among six women undergoing in vitro fertilization (IVF). We measured concentrations of 19 FF HDL particle components, including HDL cholesterol, free cholesterol, four cholesteryl esters, phospholipids, triglycerides, paraoxonase and arylesterase activities, apolipoproteins A-1 and A-2 (ApoA-1 and ApoA-2), and seven lipophilic micronutrients, by automated analysis and with high-performance liquid chromatography. We assessed biological variability using two-stage nested analysis of variance and compared values with those previously published for contralateral follicles. RESULTS: For most FF HDL analytes, there was little variability between follicles relative to the variability between women (i.e., %σ(2) F:%σ(2) B <0.5). Intraclass correlation coefficients were >0.80 for HDL cholesterol (0.82), phospholipids (0.89), paraoxonase (0.96), and arylesterase (0.91) activities, ApoA-1 (0.89), and ApoA-2 (0.90), and single specimen collections were required to estimate the subject-specific mean, demonstrating sufficient reliability for use as biomarkers of the follicular microenvironment in epidemiologic and clinical studies. CONCLUSIONS: These preliminary results raise the possibility for tighter regulation of HDL in follicles within the same ovary vs. between ovaries. Thus, collection of a single FF specimen may be sufficient to estimate HDL particle components concentrations within a single ovary. However, our results should be interpreted with caution as the analysis was based on a small sample.
Entities:
Keywords:
Biological variability; Follicular fluid (FF); High density lipoprotein (HDL); Human ovarian follicles; In vitro fertilization (IVF)
Authors: Richard W Browne; Stephen T Koury; Susan Marion; Gregory Wilding; Paola Muti; Maurizio Trevisan Journal: Clin Chem Date: 2006-12-21 Impact factor: 8.327
Authors: Eleonora B Pasqualotto; Ashok Agarwal; Rakesh K Sharma; Vicente M Izzo; José A Pinotti; Narendra J Joshi; Bruce I Rose Journal: Fertil Steril Date: 2004-04 Impact factor: 7.329
Authors: Y J Liu; B Vlaeminck; K Tilleman; P Y Chouinard; R Gervais; P De Sutter; V Fievez Journal: J Assist Reprod Genet Date: 2020-01-18 Impact factor: 3.412