Literature DB >> 26757811

A simple, low-cost staining method for rapid-throughput analysis of tumor spheroids.

Frank Eckerdt1, Angel Alvarez2, Jonathan Bell1, Constadina Arvanitis3, Asneha Iqbal1,4, Ahmet D Arslan1, Bo Hu2, Shi-Yuan Cheng2, Stewart Goldman1,4, Leonidas C Platanias1,5.   

Abstract

Tumor spheroids are becoming an important tool for the investigation of cancer stem cell (CSC) function in tumors; thus, low-cost and high-throughput methods for drug screening of tumor spheroids are needed. Using neurospheres as non-adherent three-dimensional (3-D) cultures, we developed a simple, low-cost acridine orange (AO)-based method that allows for rapid analysis of live neurospheres by fluorescence microscopy in a 96-well format. This assay measures the cross-section area of a spheroid, which corresponds to cell viability. Our novel method allows rapid screening of a panel of anti-proliferative drugs to assess inhibitory effects on the growth of cancer stem cells in 3-D cultures.

Entities:  

Keywords:  acridine orange; cancer stem cell; glioblastoma; microscopy; neurospheres; tumor spheroids

Mesh：

Substances：

Year:  2016        PMID： 26757811      PMCID： PMC4772731          DOI： 10.2144/000114372

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  12 in total

1.  384 hanging drop arrays give excellent Z-factors and allow versatile formation of co-culture spheroids.

Authors:  Amy Y Hsiao; Yi-Chung Tung; Xianggui Qu; Lalit R Patel; Kenneth J Pienta; Shuichi Takayama
Journal:  Biotechnol Bioeng       Date:  2011-12-20       Impact factor: 4.530

2.  EGFR phosphorylation of DCBLD2 recruits TRAF6 and stimulates AKT-promoted tumorigenesis.

Authors:  Haizhong Feng; Giselle Y Lopez; Chung Kwon Kim; Angel Alvarez; Christopher G Duncan; Ryo Nishikawa; Motoo Nagane; An-Jey A Su; Philip E Auron; Matthew L Hedberg; Lin Wang; Jeffery J Raizer; John A Kessler; Andrew T Parsa; Wei-Qiang Gao; Sung-Hak Kim; Mutsuko Minata; Ichiro Nakano; Jennifer R Grandis; Roger E McLendon; Darell D Bigner; Hui-Kuan Lin; Frank B Furnari; Webster K Cavenee; Bo Hu; Hai Yan; Shi-Yuan Cheng
Journal:  J Clin Invest       Date:  2014-07-25       Impact factor: 14.808

Review 3.  Assessment of fluorochromes for cellular structure and function studies by flow cytometry.

Authors:  J M Petit; M Denis-Gay; M H Ratinaud
Journal:  Biol Cell       Date:  1993       Impact factor: 4.458

4.  Correlation of the growth of Chinese hamster V279-17lb multicellular spheroids with cytokinetic parameters.

Authors:  T S Johnson; E Bain; M R Raju; J C Martin
Journal:  Cytometry       Date:  1980-07

5.  Three-Dimensional Cell Culture-Based Screening Identifies the Anthelmintic Drug Nitazoxanide as a Candidate for Treatment of Colorectal Cancer.

Authors:  Wojciech Senkowski; Xiaonan Zhang; Maria Hägg Olofsson; Ruben Isacson; Urban Höglund; Mats Gustafsson; Peter Nygren; Stig Linder; Rolf Larsson; Mårten Fryknäs
Journal:  Mol Cancer Ther       Date:  2015-04-24       Impact factor: 6.261

6.  Cancer Stem Cells: A Moving Target.

Authors:  Maria Giovanna Francipane; Julie Chandler; Eric Lagasse
Journal:  Curr Pathobiol Rep       Date:  2013-06-01

7.  The fluorometric microculture cytotoxicity assay.

Authors:  Elin Lindhagen; Peter Nygren; Rolf Larsson
Journal:  Nat Protoc       Date:  2008       Impact factor: 13.491

8.  Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation.

Authors:  Maria Vinci; Sharon Gowan; Frances Boxall; Lisa Patterson; Miriam Zimmermann; William Court; Cara Lomas; Marta Mendiola; David Hardisson; Suzanne A Eccles
Journal:  BMC Biol       Date:  2012-03-22       Impact factor: 7.431

Review 9.  Spherical cancer models in tumor biology.

Authors:  Louis-Bastien Weiswald; Dominique Bellet; Virginie Dangles-Marie
Journal:  Neoplasia       Date:  2015-01       Impact factor: 5.715

10.  Regulatory effects of a Mnk2-eIF4E feedback loop during mTORC1 targeting of human medulloblastoma cells.

Authors:  Frank Eckerdt; Elspeth Beauchamp; Jonathan Bell; Asneha Iqbal; Bing Su; Rikiro Fukunaga; Rishi R Lulla; Stewart Goldman; Leonidas C Platanias
Journal:  Oncotarget       Date:  2014-09-30
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  8 in total

1.  Potent Antineoplastic Effects of Combined PI3Kα-MNK Inhibition in Medulloblastoma.

Authors:  Frank Eckerdt; Jonathan B Bell; Elspeth M Beauchamp; Jessica Clymer; Gavin T Blyth; Ewa M Kosciuczuk; Quanhong Ma; David Z Chen; Craig Horbinski; Stewart Goldman; Hidayatullah G Munshi; Rintaro Hashizume; Leonidas C Platanias
Journal:  Mol Cancer Res       Date:  2019-03-06       Impact factor: 5.852

2.  Differential Response of Glioma Stem Cells to Arsenic Trioxide Therapy Is Regulated by MNK1 and mRNA Translation.

Authors:  Jonathan B Bell; Frank Eckerdt; Harshil D Dhruv; Darren Finlay; Sen Peng; Seungchan Kim; Barbara Kroczynska; Elspeth M Beauchamp; Kristen Alley; Jessica Clymer; Stewart Goldman; Shi-Yuan Cheng; C David James; Ichiro Nakano; Craig Horbinski; Andrew P Mazar; Kristiina Vuori; Priya Kumthekar; Jeffrey Raizer; Michael E Berens; Leonidas C Platanias
Journal:  Mol Cancer Res       Date:  2017-10-17       Impact factor: 5.852

3.  Identification and evaluation of an appropriate housekeeping gene for real time gene profiling of hepatocellular carcinoma cells cultured in three dimensional scaffold.

Authors:  Anjana Kaveri Badekila; Praveen Rai; Sudarshan Kini
Journal:  Mol Biol Rep       Date:  2021-10-19       Impact factor: 2.316

4.  MNK Inhibition Disrupts Mesenchymal Glioma Stem Cells and Prolongs Survival in a Mouse Model of Glioblastoma.

Authors:  Jonathan B Bell; Frank D Eckerdt; Kristen Alley; Lisa P Magnusson; Hridi Hussain; Yingtao Bi; Ahmet Dirim Arslan; Jessica Clymer; Angel A Alvarez; Stewart Goldman; Shi-Yuan Cheng; Ichiro Nakano; Craig Horbinski; Ramana V Davuluri; C David James; Leonidas C Platanias
Journal:  Mol Cancer Res       Date:  2016-06-30       Impact factor: 5.852

5.  HDL nanoparticles targeting sonic hedgehog subtype medulloblastoma.

Authors:  Jonathan B Bell; Jonathan S Rink; Frank Eckerdt; Jessica Clymer; Stewart Goldman; C Shad Thaxton; Leonidas C Platanias
Journal:  Sci Rep       Date:  2018-01-19       Impact factor: 4.379

6.  Combined PI3Kα-mTOR Targeting of Glioma Stem Cells.

Authors:  Frank D Eckerdt; Jonathan B Bell; Christopher Gonzalez; Michael S Oh; Ricardo E Perez; Candice Mazewski; Mariafausta Fischietti; Stewart Goldman; Ichiro Nakano; Leonidas C Platanias
Journal:  Sci Rep       Date:  2020-12-14       Impact factor: 4.379

7.  Targeting of glioblastoma cell lines and glioma stem cells by combined PIM kinase and PI3K-p110α inhibition.

Authors:  Asneha Iqbal; Frank Eckerdt; Jonathan Bell; Ichiro Nakano; Francis J Giles; Shi-Yuan Cheng; Rishi R Lulla; Stewart Goldman; Leonidas C Platanias
Journal:  Oncotarget       Date:  2016-05-31

8.  Human SLFN5 is a transcriptional co-repressor of STAT1-mediated interferon responses and promotes the malignant phenotype in glioblastoma.

Authors:  A D Arslan; A Sassano; D Saleiro; P Lisowski; E M Kosciuczuk; M Fischietti; F Eckerdt; E N Fish; L C Platanias
Journal:  Oncogene       Date:  2017-07-03       Impact factor: 9.867
  8 in total

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