Literature DB >> 2675764

Cloning and expression in Escherichia coli of the Azospirillum brasilense Sp7 gene encoding ampicillin resistance.

C Verreth1, B Cammue, P Swinnen, D Crombez, A Michielsen, K Michiels, A Van Gool, J Vanderleyden.   

Abstract

The Azospirillum brasilense ATCC 29145 gene coding for beta-lactamase was cloned in Escherichia coli. The gene was expressed in E. coli from its own promoter as a 30-kilodalton protein, conferring resistance to high levels of beta-lactam antibiotics. The DNA sequence containing the beta-lactamase gene was found to be highly amplified in the Azospirillum genome, scattered in the chromosomal as well as in the plasmidic DNA.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2675764      PMCID： PMC203002          DOI： 10.1128/aem.55.8.2056-2060.1989

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  17 in total

1.  ["Beta-lactamase enzymogram": an agar adaptation of the iodometric method (author's transl)].

Authors:  R Labia; M Barthélémy
Journal:  Ann Microbiol (Paris)       Date:  1979-10

2.  A complementation analysis of the restriction and modification of DNA in Escherichia coli.

Authors:  H W Boyer; D Roulland-Dussoix
Journal:  J Mol Biol       Date:  1969-05-14       Impact factor: 5.469

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Use of minicells for bacteriophage-directed polypeptide synthesis.

Authors:  J Reeve
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

5.  Origin of the TEM-beta-lactamase gene found on plasmids.

Authors:  F Heffron; R Sublett; R W Hedges; A Jacob; S Falkow
Journal:  J Bacteriol       Date:  1975-04       Impact factor: 3.490

6.  Rapid procedure for detection and isolation of large and small plasmids.

Authors:  C I Kado; S T Liu
Journal:  J Bacteriol       Date:  1981-03       Impact factor: 3.490

7.  Mutagenesis by insertion of the drug resistance transposon Tn7 applied to the Ti plasmid of Agrobacterium tumefaciens.

Authors:  J P Hernalsteens; H De Greve; M Van Montagu; J Schell
Journal:  Plasmid       Date:  1978-02       Impact factor: 3.466

8.  Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors:  F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal:  Gene       Date:  1977       Impact factor: 3.688

9.  Physiological properties and plasmid content of several strains of Azospirillum brasilense and A. lipoferum.

Authors:  C Franche; C Elmerich
Journal:  Ann Microbiol (Paris)       Date:  1981 Jan-Feb

10.  Isolation of supercoiled colicinogenic factor E 1 DNA sensitive to ribonuclease and alkali.

Authors:  D G Blair; D J Sherratt; D B Clewell; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1972-09       Impact factor: 11.205
View more
  2 in total

1.  β-Lactam Resistance in Azospirillum baldaniorum Sp245 Is Mediated by Lytic Transglycosylase and β-Lactamase and Regulated by a Cascade of RpoE7→RpoH3 Sigma Factors.

Authors:  Parul Pandey; Ashutosh P Dubey; Shivangi Mishra; Vijay Shankar Singh; Chhaya Singh; Anil K Tripathi
Journal:  J Bacteriol       Date:  2022-03-30       Impact factor: 3.476

2.  An energy taxis transducer promotes root colonization by Azospirillum brasilense.

Authors:  Suzanne E Greer-Phillips; Bonnie B Stephens; Gladys Alexandre
Journal:  J Bacteriol       Date:  2004-10       Impact factor: 3.490
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.