Abbas Ali Gaeini1, Leila Shafiei Neek1, Siroos Choobineh1, Mohamadreza Baghaban Eslaminejad2, Sadegh Satarifard1, Forough Azam Sayahpour2, Seyedeh Neda Mousavi3. 1. a Department of Sport Physiology , Faculty of Physical Education and Sport Sciences, University of Tehran , Tehran , Iran . 2. b Department of Stem Cells and Developmental Biology , Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR , Tehran , Iran , and. 3. c Department of Cellular and Molecular Nutrition , School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences , Tehran , Iran.
Abstract
OBJECTIVE: In this study, we investigate the effects of preconception endurance training with or without voluntary exercise during pregnancy on indices of bone formation and resorption in female offspring bone. METHODS: Twenty-four C57BL/6 female mice were randomly divided into four groups: trained in preconception period and exercised during pregnancy (TE); trained in preconception periods but unexercised during pregnancy (TC); untrained in preconception periods but exercised during pregnancy (CE); untrained and unexercised (CC). Trained dams were subjected to a protocol of moderate exercise training over a period of 4 weeks before pregnancy. Analyses were performed on the adult female offspring that did not have access to running wheels in any portion of their lives. RESULTS: The OPG, Runx2, COLI, ALP, and OPN mRNA expression was significantly up-regulated in offspring born to dams that was trained in preconception period. However, there was no significant difference in OPG, COLI, Runx2, and ALP expression in TE and TC offspring (p > 0.05). RANKL and osteocalcin expression were significantly down-regulated in TE offspring group (p < 0.001). CONCLUSIONS: Improved physical fitness in preconception period results in significant changes in bone gene expressions of female offspring, in particular towards osteogenic responses with improved RANKL/OPG ratio.
OBJECTIVE: In this study, we investigate the effects of preconception endurance training with or without voluntary exercise during pregnancy on indices of bone formation and resorption in female offspring bone. METHODS: Twenty-four C57BL/6 female mice were randomly divided into four groups: trained in preconception period and exercised during pregnancy (TE); trained in preconception periods but unexercised during pregnancy (TC); untrained in preconception periods but exercised during pregnancy (CE); untrained and unexercised (CC). Trained dams were subjected to a protocol of moderate exercise training over a period of 4 weeks before pregnancy. Analyses were performed on the adult female offspring that did not have access to running wheels in any portion of their lives. RESULTS: The OPG, Runx2, COLI, ALP, and OPN mRNA expression was significantly up-regulated in offspring born to dams that was trained in preconception period. However, there was no significant difference in OPG, COLI, Runx2, and ALP expression in TE and TC offspring (p > 0.05). RANKL and osteocalcin expression were significantly down-regulated in TE offspring group (p < 0.001). CONCLUSIONS: Improved physical fitness in preconception period results in significant changes in bone gene expressions of female offspring, in particular towards osteogenic responses with improved RANKL/OPG ratio.
Entities:
Keywords:
Bone; offspring; physical fitness; preconception period
Authors: Jenney Liu; Icksoo Lee; Han-Zhong Feng; Sujay S Galen; Philipp P Hüttemann; Guy A Perkins; J-P Jin; Maik Hüttemann; Moh H Malek Journal: J Strength Cond Res Date: 2018-05 Impact factor: 3.775