Literature DB >> 26753520

Ultrasensitive determination of 5-methylcytosine and 5-hydroxymethylcytosine in genomic DNA by sheathless interfaced capillary electrophoresis-mass spectrometry.

Fang Yuan1, Xiao-Hui Zhang1, Ji Nie1, Hong-Xu Chen2, Ying-Lin Zhou1, Xin-Xiang Zhang1.   

Abstract

A newly developed sheathless interface for capillary electrophoresis-mass spectrometry, using a porous tip sprayer, was first applied for highly sensitive determination of cytosine modifications. The system performed well in identification and quantification of both 5-methylcytosine and 5-hydroxymethylcytosine using only 125 pg (∼20 cells) genomic DNA samples.

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Year:  2016        PMID： 26753520     DOI： 10.1039/c5cc10155g

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  5 in total

Review 1.  Formation and repair of oxidatively generated damage in cellular DNA.

Authors:  Jean Cadet; Kelvin J A Davies; Marisa Hg Medeiros; Paolo Di Mascio; J Richard Wagner
Journal:  Free Radic Biol Med       Date:  2017-01-02       Impact factor: 7.376

2.  Bisulfite-free and single-nucleotide resolution sequencing of DNA epigenetic modification of 5-hydroxymethylcytosine using engineered deaminase.

Authors:  Neng-Bin Xie; Min Wang; Tong-Tong Ji; Xia Guo; Jiang-Hui Ding; Bi-Feng Yuan; Yu-Qi Feng
Journal:  Chem Sci       Date:  2022-05-26       Impact factor: 9.969

Review 3.  Single Cell Neurometabolomics.

Authors:  Meng Qi; Marina C Philip; Ning Yang; Jonathan V Sweedler
Journal:  ACS Chem Neurosci       Date:  2017-10-19       Impact factor: 4.418

4.  5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers.

Authors:  Wenshuai Li; Xu Zhang; Xingyu Lu; Lei You; Yanqun Song; Zhongguang Luo; Jun Zhang; Ji Nie; Wanwei Zheng; Diannan Xu; Yaping Wang; Yuanqiang Dong; Shulin Yu; Jun Hong; Jianping Shi; Hankun Hao; Fen Luo; Luchun Hua; Peng Wang; Xiaoping Qian; Fang Yuan; Lianhuan Wei; Ming Cui; Taiping Zhang; Quan Liao; Menghua Dai; Ziwen Liu; Ge Chen; Katherine Meckel; Sarbani Adhikari; Guifang Jia; Marc B Bissonnette; Xinxiang Zhang; Yupei Zhao; Wei Zhang; Chuan He; Jie Liu
Journal:  Cell Res       Date:  2017-09-19       Impact factor: 25.617

Review 5.  CE-MS for metabolomics: Developments and applications in the period 2014-2016.

Authors:  Rawi Ramautar; Govert W Somsen; Gerhardus J de Jong
Journal:  Electrophoresis       Date:  2016-10-31       Impact factor: 3.535
  5 in total

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