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Literature DB >> 2674905

Amplification of the ribonucleotide reductase small subunit gene: analysis of novel joints and the mechanism of gene duplication in vaccinia virus.

M B Slabaugh¹, N A Roseman, C K Mathews.

Abstract

Amplification of the M2 gene encoding the small subunit of ribonucleotide reductase (EC 1.17.4.1) was analyzed in a collection of vaccinia virus (VV) isolates selected for resistance to 5 mM hydroxyurea (HU). Most of the mutants harbored tandem direct repeat arrays of the M2 gene, but several had duplicated M2 as an inverted repeat by genomic rearrangements involving the chromosomal termini. Novel joints formed by direct repeats were mapped, amplified in vitro, and sequenced. The junctions were simple fusions between DNA downstream and upstream of the M2 gene. Lack of sequence homology at the breakpoints indicated that the initial genomic rearrangements leading to gene amplification were due to nonhomologous recombination events.

Entities: Disease Species

Mesh：

Substances：

Year: 1989 PMID： 2674905 PMCID： PMC318434 DOI： 10.1093/nar/17.17.7073

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

34 in total

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Journal: Virology Date: 1981-03 Impact factor: 3.616

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Authors: K C Reed; D A Mann
Journal: Nucleic Acids Res Date: 1985-10-25 Impact factor: 16.971

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Authors: J Nalbantoglu; D Hartley; G Phear; G Tear; M Meuth
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19 in total

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4. Structure and organization of amplicons containing the E4 esterase genes responsible for insecticide resistance in the aphid Myzus persicae (Sulzer).

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Authors: M B Slabaugh; M L Howell; Y Wang; C K Mathews
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6. Experimental Evolution Identifies Vaccinia Virus Mutations in A24R and A35R That Antagonize the Protein Kinase R Pathway and Accompany Collapse of an Extragenic Gene Amplification.

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