Yuko Saito1, Ayako Shioya1,2, Terunori Sano1, Hiroyuki Sumikura3,4, Miho Murata5, Shigeo Murayama3,4. 1. Department of Pathology and Laboratory Medicine, National Center Hospital, National Center of Neurology and Psychiatry, Tokyo, Japan. 2. Department of Neurology, Graduate School of Comprehensive Human Science, University of Tsukuba, Ibaraki, Japan. 3. Departments of Neuropathology and Neurology, Tokyo Metropolitan Institute of Gerontology, Itabashi, Tokyo, Japan. 4. Department of Neurology, Graduate School of Medicine, Osaka University, Osaka, Japan. 5. Department of Neurology, National Center Hospital, National Center of Neurology and Psychiatry, Tokyo, Japan.
Abstract
BACKGROUND: The "dual-hit" and propagation hypotheses of α-synuclein suggests that the olfactory cells of the olfactory epithelium are among the earliest sites of involvement in Parkinson's disease (PD). We investigated the olfactory epithelium in consecutive cases that had been registered with a brain bank. OBJECTIVES: This study was undertaken to check the presence or absence of Lewy body pathology in olfactory cells. METHODS: Thirty-six male and 11 female patients were examined, including eight with PD, two with dementia with Lewy bodies, 11 with incidental Lewy body disease, and 26 with no Lewy-related alpha-synucleinopathy. The olfactory epithelium was sampled by craniotomy followed by resection of the cribriform plate, which was fixed in formalin and decalcified with ethylenediaminetetra-acetate. Coronal paraffin-embedded sections of the plate were stained with hematoxylin and eosin or immunohistochemically stained with antibodies against phosphorylated α-synuclein to detect Lewy body pathology and neuronal markers of protein gene product 9.5, phosphorylated neurofilament, and tyrosine hydroxylase. RESULTS: Lewy body pathology was detected in the olfactory cells of the olfactory epithelium in a single patient with incidental Lewy body disease and in six patients with PD, but it was not detected in patients who had dementia with Lewy bodies. CONCLUSIONS: We detected Lewy body pathology in the olfactory epithelium in six of the eight patients with Parkinson's disease and in one patient with incidental Lewy body pathology.
BACKGROUND: The "dual-hit" and propagation hypotheses of α-synuclein suggests that the olfactory cells of the olfactory epithelium are among the earliest sites of involvement in Parkinson's disease (PD). We investigated the olfactory epithelium in consecutive cases that had been registered with a brain bank. OBJECTIVES: This study was undertaken to check the presence or absence of Lewy body pathology in olfactory cells. METHODS: Thirty-six male and 11 female patients were examined, including eight with PD, two with dementia with Lewy bodies, 11 with incidental Lewy body disease, and 26 with no Lewy-related alpha-synucleinopathy. The olfactory epithelium was sampled by craniotomy followed by resection of the cribriform plate, which was fixed in formalin and decalcified with ethylenediaminetetra-acetate. Coronal paraffin-embedded sections of the plate were stained with hematoxylin and eosin or immunohistochemically stained with antibodies against phosphorylated α-synuclein to detect Lewy body pathology and neuronal markers of protein gene product 9.5, phosphorylated neurofilament, and tyrosine hydroxylase. RESULTS: Lewy body pathology was detected in the olfactory cells of the olfactory epithelium in a single patient with incidental Lewy body disease and in six patients with PD, but it was not detected in patients who had dementia with Lewy bodies. CONCLUSIONS: We detected Lewy body pathology in the olfactory epithelium in six of the eight patients with Parkinson's disease and in one patient with incidental Lewy body pathology.
Authors: Christian Pifl; Harald Reither; Natalia Lopez-Gonzalez Del Rey; Carmen Cavada; Jose A Obeso; Javier Blesa Journal: Front Neuroanat Date: 2017-05-29 Impact factor: 3.856
Authors: Bolek Zapiec; Birger V Dieriks; Sheryl Tan; Richard L M Faull; Peter Mombaerts; Maurice A Curtis Journal: Brain Date: 2017-10-01 Impact factor: 13.501