Lorenzo Drago1, Laura Cappelletti2, Claudio Lamartina3, Pedro Berjano4, Roberto Mattina5, Elena De Vecchi2. 1. Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopaedic Institute, Milan Italy; Laboratory of Microbiology and Technical Sciences for Laboratory Medicine, Department of Biomedical Sciences for Health, University of Milan, Milan Italy. Electronic address: lorenzo.drago@unimi.it. 2. Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopaedic Institute, Milan Italy. 3. 2(nd) Spine Surgery Division, IRCCS Galeazzi Orthopaedic Institute, Milan Italy. 4. 4(th) Spine Surgery Division, IRCCS Galeazzi Orthopaedic Institute, Milan Italy. 5. Department of Biomedical, Surgical and Odontoiatric Sciences, University of Milan, Milan.
Abstract
INTRODUCTION: Staphylococcus aureus and coagulase-negative staphylococci (CoNS) colonization among healthcare workers (HCWs) may have implications in development of infections and in spreading of resistance. This study aimed to determine the rate of methicillin-resistant staphylococci carriage in HCWs of spinal surgeries in an Italian Orthopaedic Institute. MATERIALS AND METHODS: Samples from nares, axillae and hands were inoculated onto appropriate media in order to perform colony counts of methicillin-susceptible and resistant S. aureus and CoNS. RESULTS: Prevalence of S. aureus and CNS was 42.3% and 98%, respectively. Methicillin-resistance was rather infrequent in S. aureus (13.5%) while it was detected in most of CoNS (90.4%). Methicillin resistant S. aureus were prevalently isolated from nares while axillae showed the highest methicillin-resistant CoNS colonization rates. CONCLUSIONS: A relatively high rate of methicillin resistant staphylococci was found among HCWs in spinal surgeries wards, thus evidencing the need for careful prevention measures and for periodic evaluation of spread among HCWs.
INTRODUCTION:Staphylococcus aureus and coagulase-negative staphylococci (CoNS) colonization among healthcare workers (HCWs) may have implications in development of infections and in spreading of resistance. This study aimed to determine the rate of methicillin-resistant staphylococci carriage in HCWs of spinal surgeries in an Italian Orthopaedic Institute. MATERIALS AND METHODS: Samples from nares, axillae and hands were inoculated onto appropriate media in order to perform colony counts of methicillin-susceptible and resistant S. aureus and CoNS. RESULTS: Prevalence of S. aureus and CNS was 42.3% and 98%, respectively. Methicillin-resistance was rather infrequent in S. aureus (13.5%) while it was detected in most of CoNS (90.4%). Methicillin resistant S. aureus were prevalently isolated from nares while axillae showed the highest methicillin-resistant CoNS colonization rates. CONCLUSIONS: A relatively high rate of methicillin resistant staphylococci was found among HCWs in spinal surgeries wards, thus evidencing the need for careful prevention measures and for periodic evaluation of spread among HCWs.
Authors: Michał Michalik; Alfred Samet; Adrianna Podbielska-Kubera; Vincenzo Savini; Jacek Międzobrodzki; Maja Kosecka-Strojek Journal: Ann Clin Microbiol Antimicrob Date: 2020-06-04 Impact factor: 3.944