| Literature DB >> 26741856 |
Ines Pickrahn1, Eva Müller1, Waltraud Zahrer1, Bettina Dunkelmann1, Jan Cemper-Kiesslich1, Gabriele Kreindl1, Franz Neuhuber2.
Abstract
With the new 6-dye AmpFISTR(®) Yfiler(®) Plus amplification kit (Thermo Fisher Scientific, Waltham, MA, USA) a set of 25 Y-chromosomal short tandem repeat loci (Y-STRs), including seven rapidly mutating Y-STRs (RM Y-STRs), is now available for forensic DNA typing. In this study we present our validation data for the AmpFISTR(®) Yfiler(®) Plus amplification kit and show the results of Y-chromosomal typing of 425 unrelated male individuals from two Austrian populations (Salzburg and Upper Austria) with the AmpFISTR(®) Yfiler(®) Plus amplification kit. Forensic parameters were calculated and compared for four Y-STR marker sets. We also typed five brother pairs to evaluate the power of discrimination for related individuals. The AmpFISTR(®) Yfiler(®) Plus (Yfiler Plus) kit appeared to be unimpaired by typical inhibitors such as hematin and humic acid or by large amounts of female components. An upgrade of analyzed markers resulted in increased discrimination capacity that is crucial for forensic trace analysis.Entities:
Keywords: Haplotype diversity; Rapidly mutating Y-STRs (RM Y-STRs); Y-STRs; Y-chromosome; Yfiler; Yfiler Plus
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Year: 2015 PMID： 26741856 DOI： 10.1016/j.fsigen.2015.12.014
Source DB: PubMed Journal: Forensic Sci Int Genet ISSN： 1872-4973 Impact factor: 4.882