Roberta El Hariri Viana1, Simone G dos Santos2, Adriana C Oliveira3. 1. Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. 2. Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. 3. Department of Basic Nursing, School of Nursing, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Electronic address: adrianacoliveira@gmail.com.
Abstract
BACKGROUND: Microorganisms may contaminate hospital mattresses even after terminal cleaning. We investigated the recovery of resistant bacteria from the mattresses of patients under contact precautions at a university hospital. METHODS: We conducted a cross-sectional study. Samples were obtained from the surface of mattresses, spread on replicate organism detection and counting plates, and cultivated at 37°C for 48 hours. After collecting samples, we identified microorganisms and tested for antimicrobial susceptibility using the Vitek 2 (bioMérieux SA, Marcy-l'Etoile, France) automation system. RESULTS: We evaluated 51 mattresses. A total of 26 had resistant bacteria on the surface; the predominant species were Acinetobacter baumannii (69.2%), Klebsiella pneumoniae (11.5%), and Pseudomonas aeruginosa (11.5%). The median length of hospital stay was 41 days; the bed occupancy for patients under contact precautions and the time at which the patient was diagnosed as a carrier of resistant bacteria was 18 days. CONCLUSIONS: The phenotypic similarity of A baumannii in inpatient units (mattresses) suggests circulation of the same strain. These results highlight the importance of controlling the potential spread of microorganisms through hospital mattresses.
BACKGROUND: Microorganisms may contaminate hospital mattresses even after terminal cleaning. We investigated the recovery of resistant bacteria from the mattresses of patients under contact precautions at a university hospital. METHODS: We conducted a cross-sectional study. Samples were obtained from the surface of mattresses, spread on replicate organism detection and counting plates, and cultivated at 37°C for 48 hours. After collecting samples, we identified microorganisms and tested for antimicrobial susceptibility using the Vitek 2 (bioMérieux SA, Marcy-l'Etoile, France) automation system. RESULTS: We evaluated 51 mattresses. A total of 26 had resistant bacteria on the surface; the predominant species were Acinetobacter baumannii (69.2%), Klebsiella pneumoniae (11.5%), and Pseudomonas aeruginosa (11.5%). The median length of hospital stay was 41 days; the bed occupancy for patients under contact precautions and the time at which the patient was diagnosed as a carrier of resistant bacteria was 18 days. CONCLUSIONS: The phenotypic similarity of A baumannii in inpatient units (mattresses) suggests circulation of the same strain. These results highlight the importance of controlling the potential spread of microorganisms through hospital mattresses.
Authors: Micaela Machado Querido; Lívia Aguiar; Paula Neves; Cristiana Costa Pereira; João Paulo Teixeira Journal: Colloids Surf B Biointerfaces Date: 2019-02-16 Impact factor: 5.268