Literature DB >> 26738688

Multiplex RT-PCR detection of H3N2 influenza A virus in dogs.

EunJung Lee1, Eun-Ju Kim1, Bo-Hye Kim1, Jae-Young Song2, In-Soo Cho1, Yeun-Kyung Shin3.   

Abstract

A multiplex RT-PCR (mRT-PCR) assay to detect H3N2 CIV genomic segments was developed as a rapid and cost-effective method. Its performance was evaluated with forty-six influenza A viruses from different hosts using three primer sets which amplify four segments of H3N2 CIV simultaneously. The mRT-PCR has been successful in detecting the viral segments, indicating that it can improve the speed of diagnosis for H3N2 CIV and its reassortants.
Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.

Entities:  

Keywords:  H3N2 CIV; Multiplex RT-PCR; Reassortants

Mesh:

Substances:

Year:  2015        PMID: 26738688     DOI: 10.1016/j.mcp.2015.12.002

Source DB:  PubMed          Journal:  Mol Cell Probes        ISSN: 0890-8508            Impact factor:   2.365


  1 in total

1.  A Multiplex RT-PCR Assay for Detection and Differentiation of Avian-Origin Canine H3N2, Equine-Origin H3N8, Human-Origin H3N2, and H1N1/2009 Canine Influenza Viruses.

Authors:  Chenxi Wang; Qian Wang; Junyi Hu; Honglei Sun; Juan Pu; Jinhua Liu; Yipeng Sun
Journal:  PLoS One       Date:  2017-01-20       Impact factor: 3.240

  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.