BACKGROUND: Increasing evidence suggests that microRNAs are widely involved in cancer progression and metastasis. However, the specific role of miR-31 in papillary thyroid carcinoma (PTC) is still largely unknown. METHODS: The level of miR-31 and HuR was detected in 30 pairedcancerous and noncancerous tissue samples using real time PCR. The impact of miR-31 on PTC cell viability and apoptosis was explored using MTT assay and flow cytometry, respectively. To explore the effect of miR-31 on HuR expression, luciferase reporter assay was used. RESULTS: In papillary thyroid carcinoma patients, miR-31 was significantly down regulated. Furthermore, down regulation of miR-31 increased the proliferation, migration, and invasion of ovarian carcinoma cells. Vice versa, over expression of miR-31 repressed cell invasion and viability. The luciferase reporter assay revealed that HuR was a target for miR-31. Further analysis defined that knockdown of HuR resulted in enhanced cell viability and decreased cell migration rate. CONCLUSIONS: Down regulation of miR-31 contributed to the malignant progression of papillary thyroid carcinoma cells by targeting HuR.
BACKGROUND: Increasing evidence suggests that microRNAs are widely involved in cancer progression and metastasis. However, the specific role of miR-31 in papillary thyroid carcinoma (PTC) is still largely unknown. METHODS: The level of miR-31 and HuR was detected in 30 pairedcancerous and noncancerous tissue samples using real time PCR. The impact of miR-31 on PTC cell viability and apoptosis was explored using MTT assay and flow cytometry, respectively. To explore the effect of miR-31 on HuR expression, luciferase reporter assay was used. RESULTS: In papillary thyroid carcinomapatients, miR-31 was significantly down regulated. Furthermore, down regulation of miR-31 increased the proliferation, migration, and invasion of ovarian carcinoma cells. Vice versa, over expression of miR-31 repressed cell invasion and viability. The luciferase reporter assay revealed that HuR was a target for miR-31. Further analysis defined that knockdown of HuR resulted in enhanced cell viability and decreased cell migration rate. CONCLUSIONS: Down regulation of miR-31 contributed to the malignant progression of papillary thyroid carcinoma cells by targeting HuR.
Authors: Howell F Moffett; Adam N R Cartwright; Hye-Jung Kim; Jernej Godec; Jason Pyrdol; Tarmo Äijö; Gustavo J Martinez; Anjana Rao; Jun Lu; Todd R Golub; Harvey Cantor; Arlene H Sharpe; Carl D Novina; Kai W Wucherpfennig Journal: Nat Immunol Date: 2017-05-22 Impact factor: 25.606
Authors: Maria Szubert; Anna Nowak-Glück; Daria Domańska-Senderowska; Bożena Szymańska; Piotr Sowa; Aleksander Rycerz; Jacek R Wilczyński Journal: Int J Mol Sci Date: 2022-04-22 Impact factor: 6.208